Cardioprotective effects of Pinus eldarica bark extract on adrenaline-induced myocardial infarction in rats

Abstract

Objective: To investigate the effect of Pinus eldarica bark extract on adrenaline-induced myocardial infarction. Methods: Hydroalcoholic extract was prepared using maceration method and its total phenolic content was determined using the Folin-ciocalteu method. Pretreatment was done by oral administration of 100, 200, and 400 mg/kg Pinus eldarica bark extract for 16 days in male Wistar rats. Injection of adrenaline (2 mg/kg, s.c.) was performed on the 15th and 16th days for induction of myocardial infarction. Lead II ECG was recorded. Serum cardiac marker enzymes and antioxidative parameters were evaluated and a histopathological examination of heart tissues was performed. Results: Pretreatment with Pinus eldarica bark extract especially at its high doses significantly lowered the ST-segment elevation, improved heart rate, and decreased RR interval in ECG pattern of rats with adrenaline-induced myocardial infarction. It declined serum markers of heart damage including aspartate aminotransferase, lactate dehydrogenase, and creatine phosphokinase-MB, and also decreased lipid peroxidation marker, and heart weight while raising total antioxidant capacity and considerably improved histopathological alterations of the heart induced by adrenaline. Conclusions: Pinus eldarica bark extract shows beneficial cardioprotective and antioxidant effects against adrenaline-induced myocardial infarction. It can be further explored as a potential treatment for myocardial infarction.

Keywords

Adrenaline / Antioxidant / Lipid peroxidation / Myocardial infarction / Pinus eldarica

Conflict of interest statement

The authors have no conflict of interest to declare.

Funding

This study was financially supported by Vice-Chancellery for Research and Technology of Isfahan University of Medical Sciences (research projects No. 3400680).

Authors’ contributions

LS was responsible for the conceptualization of the study, supervision, designing the animal investigation, and editing of the manuscript; ZH contributed to the histopathological examination; BZ designed the herbal studies; MA was involved in the animal treatments, data acquisition, and preparation of the manuscript.

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