Textile dyeing wastewater negatively influences the hematological profile and reproductive health of male Swiss albino mice

Abstract

Objective: To determine the effects of textile dyeing industrial wastewater on the hematological parameters and reproductive health including histoarchitecture of male gonad (testes) of mice.

Methods: Twenty-four Swiss albino mice at 4-weeks old were divided into four groups (n=6 per group). Mice of group 1 supplied with normal drinking water were served as the control group. Mice of group 2, 3 and 4 were supplied normal drinking water mixed with textile dyeing wastewater at 5%, 10% and 20% concentration, respectively. After completing 24 weeks of treatment, different hematological profile, weight of testes, gonadosomatic index (GSI), sperm concentration and morphology were measured. Moreover, histopathological changes in testes were examined.

Results: Hematocrit value and hemoglobin concentrations were decreased in all groups of wastewater-treated mice compared to the control group. Likewise, weight of testes, GSI and sperm concentration were decreased significantly in wastewater-treated mice in comparison to the control group. The percentage of morphologically healthy epididymal sperm was significantly reduced in wastewater-treated mice. Histopathological examination revealed degenerative changes in seminiferous tubules, a smaller number of spermatogenic cells, elongation of seminiferous tubules and degenerative changes of seminiferous tubules in wastewater- treated mice.

Conclusions: Textile dyeing wastewater has harmful effects on hematological profile and reproductive health of male mice.

Keywords

Textile dyeing wastewater / Gonads / Hematology / Histopathology / Mice

Conflict of interest statement

Declared conflicts of interest do not exist for the authors. There are no financial conflicts to disclose, and all co-authors have reviewed and approved the manuscript's content.

Acknowledgements

Sincere gratitude to the Head, laboratory animal research divisions, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR'B) for providing the mice.

Funding

This study was funded by the Ministry of Science and Technology of the Government of People’s Republic of Bangladesh (163- BS/2020-2021).

Data availability statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Authors’ contributions

Md. Taimur Islam conceived the idea, designed the experiments, performed laboratory experiment and drafted the first version of the manuscript; Nusrat Binte Rafique, Minhaz Ahmed, Md. Mizanur Rahman and Mohosina Mou performed the sample collection and laboratory experiment; Dipu Roy and Robius Sani Sadi edited the manuscript; Anup Kumar Talukder participated in the idea development, data analysis and edited the manuscript; Ziban Chandra Das and Md. Golam Haider reviewed the manuscript. All authors have read and approved the final version of the manuscript.

Publisher’s Note

The Publisher of the Journal remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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