Methanolic pomegranate dried peel extract improves cryopreserved semen quality and antioxidant capacity of rams

Abstract

Objective: To select the appropriate concentrations of methanolic pomegranate extract supplemented in rams' semen extender for obtaining the best-cryopreserved semen quality.

Methods: Tris-based semen extender was supplemented with 0.0, 0.40, 0.48, and 0.56 mg/mL pomegranate peel methanolic extract to extend semen collected from five native rams twice weekly for two months (n=80). Pooled (n=16) post-thaw semen characteristics were determined. Thawed seminal plasma of all supplemented and control groups were used to measure malondialdehyde (MDA), nitric oxide (NO), glutathione peroxidase (GPx), superoxide dismutase (SOD), ascorbic acid, zinc, copper, total cholesterol, low-density lipoproteins (LDL), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP).

Results: The supplementation of Tris-based semen extender with 0.48 mg/mL semen extender resulted in the highest post-thaw sperm total motility (P<0.001), sperm progressive motility (P<0.001), live sperm (P<0.001), sperm plasma membrane integrity (P<0.001), acrosome integrity (P<0.001), SOD (P<0.05), zinc (P<0.001), total cholesterol (P<0.001), and LDL (P<0.001) with the lowest percentage of abnormal sperm morphology (P<0.001), the lowest lipid peroxidation (MDA, P<0.01), ascorbic acid (P>0.05), and LDH (P>0.05).

Conclusions: Pomegranate peel methanolic extract 0.48 mg/mL supplemented to Tris-based semen extender of rams is the best enrichment in preserving the sperm post-thaw characteristics via improving biochemical profiles and antioxidant capacity.

Keywords

Oxidants-antioxidants / Pomegranate peel ethanolic extract / Post-thaw semen characteristics / Tris-based semen diluents / Rams

Conflict of interest statement

The authors declare that they do not have any conflict of interest.

Funding

This study received no extramural funding.

Authors' contributions

Amai M. Aboelmaaty performed the clinical analysis, made the statistical analysis, helped in writing the manuscript and submission for publication. Mohamed S. Kotp helped in clinical and statistical analysis. Islam E. El-Seadawy collected the fruits, prepared the extract, and characterized the phenolic composition and the scavenging properties and helped in writing the manuscript. Aya M. Fadl, Elshymaa A. Abdelnaby, and Hossam R. El-Sherbiny collected semen and evaluated it, prepared the semen extenders and defined the concentrations, cryopreserved semen, and analyzed the post-thaw semen characteristics. The manuscript has been read and approved by all the authors.

Publisher’ s Note

The Publisher of the Journal remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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