Background: Currently, there is no imaging-based method for directly detecting active diastolic dysfunction. This study aimed to evaluate the efficacy of isovolumic relaxation strain imaging (IVSI) in assessing active diastolic dysfunction in preclinical settings.
Methods: Active diastolic function was assessed in C57BL/6J mice subjected to transverse aortic constriction (TAC) or a sham operation using daily conventional echocardiography and strain imaging over a 14-day period, with follow-ups at weeks 4 and 8. A modified approach was developed to accurately identify the isovolumic relaxation time (IVRT) using an apical three-chamber view.
Results: The novel imaging protocol successfully identified IVRT in each mouse. TAC mice exhibited significant alterations in E/A and E/E′ ratios from day 12 to 14, while the average strain rate detected by IVSI showed a significant decrease from day 4. After 8 weeks, TAC mice developed severe heart failure with reduced ejection fraction, but conventional echocardiography failed to detect diastolic dysfunction.
Conclusion: IVSI continued to indicate obvious diastolic alterations. Together, these data suggested that IVSI is an effective imaging-based method for direct detection of active diastolic dysfunction with high accuracy and sensitivity.
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2026 The Author(s). Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences.
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