A mouse model of lung ischemia–reperfusion injury with reversible left hilar entrapment

Abstract

Lung ischemia–reperfusion injury (LIRI), an acute lung injury syndrome triggered by lung transplantation or distal organ ischemia, has long been a difficult and hot issue in clinical research. In this study, we proposed a simple and less invasive reversible LIRI surgical protocol, achieved by improving the mouse left hilar entrapment model, which significantly improved the operability of the experiment and the reproducibility of the results. The protocol achieves precise control of the ischemic and reperfusion processes by visualizing transoral intubation, using reversible ligation of live knots to clamp the left hilar, and temporary closure of the thoracic cavity during ischemia. The reversible survival model we constructed not only provides a reliable tool to study the cellular and molecular mechanisms of LIRI but also can be used to assess the stage of injury regression, experimental pneumonia, and survival in mice. In addition, it simplifies the lung portal separation clamping operation for reversible clamping and provides an easy-to-learn visual tracheal intubation method that can be quickly mastered and replicated by beginners for consistent and reliable results.

Keywords

lung ischemia–reperfusion injury / reversible clamping / visual tracheal intubation

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2025 The Author(s). Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences.