Antibacterial effects of lidocaine and saline irrigation on Escherichia coli in superficial surgical wounds

Abstract

This study evaluated the antibacterial effects of 2% lidocaine and its combination with 0.9% saline solution on Escherichia coli infection in superficial surgical wounds in Wistar rats. The goal was to determine if these treatments could effectively reduce E. coli Colony Forming Units (CFUs) below the critical threshold of 1 × 10⁵. Seventy male Wistar rats were divided into seven groups, each undergoing different interventions to assess the antibacterial efficacy of lidocaine, with outcomes measured through bacterial cultures and CFU quantification. Results demonstrated a Log₁₀ reduction of approximately 0.44 in E. coli CFUs following infiltration with 2% lidocaine. The combined use of 2% lidocaine infiltration and 0.9% saline irrigation resulted in nearly complete suppression of bacterial growth. These findings suggest that these simple interventions could be valuable in emergency surgical settings to mitigate the risk of surgical site infections and serve as effective prophylactic measures.

Keywords

antibacterial / bacterial development / immune system / infection / surgical infection site

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2025 The Author(s). Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences.