Characterization and antibacterial/cytotoxic activity of silver nanoparticles synthesized from Dicranum scoparium moss extracts growing in Armenia

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Abstract

This study focuses on a simple, non-toxic, and environmentally friendly method for the green synthesis of silver nanoparticles using Dicranum scoparium moss extract. It includes the characterization of the biosynthesized nanoparticles and an evaluation of their antibacterial, antifungal, and anticancer activities. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) analyses confirmed that the biosynthesized silver nanoparticles were within the nanoscale range (50–100 nm) and exhibited an irregular morphology. The biogenic nanoparticles demonstrate antibacterial activity against bacterial strains Staphylococcus aureus, Bacillus mesentericus, Escherichia coli, and Pseudomonas aeruginosa. The results indicate a pronounced antibacterial activity against E. coli and P. aeruginosa compared to the tested Gram-positive bacteria, which is attributed to differences in the bacterial cell wall structure. Additionally, the green synthesized silver nanoparticles inhibited the growth of Mucor plumber, Geotrichum candidum, Cladosporium herbarum, and Aspergillus flavus mold fungi. Additionally, they expressed considerable cytotoxic properties against cancer cells.

Keywords

Ag-NPs / green synthesis / moss Dicranium scoparium / antibacterial activity / antifungal activity

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