Changes in biophysical properties and behavior of aging human erythrocytes treated with natural polyelectrolytes

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Abstract

Performing their functions as transporters of oxygen and carbon dioxide in the body, human erythrocytes constantly circulate and are exposed to the constant influence of various substances, including nutrients, drugs, medical devices covered with coatings, etc. Therefore, we aim to investigate the biophysical behavior of erythrocytes obtained from healthy volunteers to observe their morphological type changes, alterations in the zeta potential, the electrical conductivity of the erythrocytes in suspensions, and hemolysis in percentages during cells senescence, both in presence and in absence of natural polyelectrolytes pectin (PE) and chitosan (Chi) in form of multilayer films (PEM-films). Being constructed using the layer–by–layer technique, films are an object of interest of many researchers because of their high potential to be incorporated in biomedicine. By applying optical profilometry, electrophoretic light scattering, and spectrophotometry, we tested the polyelectrolytes for any potential harm on the erythrocytes. Based on our results and the one-way analysis of variance (ANOVA) statistical analysis, we reached the conclusion that the above-mentioned polyelectrolytes were harmlessness; therefore, PE and Chi are suitable substances to implement in the clinical practice in the form of drug delivery carriers and medical devices coatings, thereby directly contacting with the human blood.

Keywords

human erythrocytes / erythrocytes senescence / pectin / chitosan

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