To clarify the specific effects of sports biomechanical regulation on improving technical performance and preventing sports injuries in rugby and soccer players, this study employed a randomized controlled trial design. A total of 48 rugby players (24 males and 24 females) and 40 soccer players (20 males and 20 females) were enrolled and randomly assigned to an experimental group (receiving an 8-week personalized biomechanical intervention) and a control group (undergoing conventional training) by gender stratification. The intervention protocol was developed and dynamically optimized based on high-precision kinematic and kinetic data collected by a multi-channel synchronous sensor system: 8 infrared high-speed motion capture cameras (sampling frequency: 200 Hz) were used to obtain the 3D motion trajectories of the athletes’ lower limb joints; 16-channel wireless surface electromyography (sEMG) sensors (sampling frequency: 1500 Hz) were applied to monitor the activation timing and amplitude of the quadriceps femoris, hamstrings, and lateral ankle muscle groups; and a 3D force platform (1000 Hz) was utilized to synchronously record ground reaction forces and lower limb joint torque data. The core of the intervention focused on optimizing the angular and torque parameters of the hip, knee, and ankle joints. Statistical analyses were performed using repeated-measures analysis of variance (ANOVA) and independent samples t-tests. The intraclass correlation coefficient (ICC) was used to assess the reliability of coaches’ scores, with ICC values ranging from 0.89 to 0.93. For technical improvement, the average skill scores of male/female rugby players in the experimental group increased from 57.83 ± 5.31 / 55.33 ± 2.87 to 68.42 ± 5.35 / 65.33 ± 3.67 (all P < 0.001); those of male/female soccer players rose from 41.85 ± 5.72 / 49.70 ± 5.13 to 58.75 ± 5.28 / 74.35 ± 6.89 (all P < 0.001). Time-frequency analysis based on sEMG sensors revealed that the percentage of myoelectric activity in key muscle groups was significantly higher in the experimental group than in the control group (e.g., a 7% increase in males and 3% in females for the lateral ankle muscle group of rugby players, P < 0.05), indicating optimized muscle activation efficiency. Regarding injury prevention, after the intervention, the hip frontal torque of female rugby players in the experimental group decreased to 8.73 ± 0.32 N·m (control group: 9.14 ± 0.41 N·m, P = 0.03), and the ankle coronal torque of male soccer players decreased by 8.6% (P = 0.02). The injury incidence during the intervention period was significantly lower in the experimental group (3.1%) than in the control group (15.6%, P = 0.01). This study confirms that multi-sensor fusion-based sports biomechanical intervention can simultaneously improve technical performance and reduce injury risk in rugby and soccer players by optimizing lower limb joint mechanical parameters and muscle activation patterns. It provides quantitative sensor data support and a precision intervention basis from an engineering perspective for the formulation of gender-specific specialized training programs.