Pediococcus sp. KB1 and rosmarinic acid cooperatively improve the symptoms in a murine cedar pollinosis by inhibition of FcεRIα via splenic increase of IL-10 and IL-27 expression

Abstract

Lactic acid bacteria and polyphenols are known to have various health-promoting effects. Pediococcus sp. KB1 (KB1) is a probiotic that was isolated from pickled suguki turnips and has been shown to have potential immunomodulatory activity and strong resistance to gastric acid. Rosmarinic acid (RA) is a phenolic compound commonly found in Lamiaceae plant species, known for its broad anti-inflammatory properties. However, the underlying mechanisms of the combined effects of KB1 and RA on pollinosis remain unclear. The aim of this study was to investigate the effects of oral administration of KB1 and RA on symptom amelioration and anti-inflammatory activity using a murine model of pollinosis. Our results showed that the KB1 + RA group had attenuated sneezing and nasal rubbing in our murine model of cedar pollinosis. The KB1 + RA group exhibited decreased serum histamine levels and reduced Cry j1-specific IgE levels. In both the KB1 and KB1 + RA groups, spleen tissue weight was significantly reduced. In addition, in the KB1 + RA group, increased mRNA expression levels of IL-10, IL-27, IFN-γ, and Foxp3 in the spleen, along with reduced FcεRI-α protein expression, were found. These observations suggest that the combined administration of KB1 + RA may alleviate symptoms by regulating the Th1/Th2 balance and anti-inflammatory cytokines. These results suggest that KB1 and RA may serve as potential natural anti-inflammatory agents for the management of pollinosis.

Keywords

Japanese cedar pollinosis / Cry j1 / heat-killed Pediococcus sp. KB1 / rosmarinic acid / anti-inflammation

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