Evaluation of conditions for the intestinal probiotic-mediated biotransformation of Sennosides into Physcion in Cassia occidentalis extracts

Abstract

Anthraquinone compounds (AQCs) are functional constituents of various medicinal and edible plants belonging to families such as Fabaceae, Polygonaceae, and Liliaceae. They include sennosides, emodin, rhein, aloe-emodin, chrysophanol, and physcion, which show physiological activities, such as bowel movement promotion, hepatoprotection, anti-inflammatory effects, and antioxidation, as well as antimicrobial properties. The seeds of Cassia occidentalis L. (Fabaceae), an annual herb widely distributed in tropical and subtropical regions, are abundant in anthraquinone compounds, of which sennosides account for approximately 80% of the total anthrone-related constituents, and have traditionally been used as stimulant laxatives. However, sennosides are inactive forms and do not exert physiological effects until they reach the colon, where they are biotransformed by intestinal microbiota into downstream metabolites with biological activity. The major bioactive sennoside metabolites are rhein anthrone and rhein, which can be detected in the mouse colon within 2–3 h after oral administration of sennosides, confirming the crucial role of gut microbiota, particularly the intestinal probiotics Bifidobacterium and Lactobacillus, in sennoside conversion. This study aimed to investigate whether commercially available common gut probiotics possess the ability to hydrolyze sennosides in C. occidentalis extracts, to determine the suitable conditions for such hydrolysis, and to further evaluate the efficiency of sennoside conversion into physcion and rhein. Of the 20 commonly used intestinal probiotic strains tested in this study, Lactobacillus delbrueckiisubsp. lactis (BCRC 14069) and Lactobacillus helveticus (BCRC 14092) were the most efficient at sennoside hydrolysis. Under suitable conversion conditions, the hydrolysis rate reached over 95%, reducing the residual sennoside content to 6–7 mg/g while significantly enhancing the production efficiency of the downstream metabolites, physcion and rhein. Furthermore, even when administered at relatively high doses according to different application needs, the hydrolyzed products remained compliant with both Taiwan Food and Drug Administration (TFDA) (12 mg/day) and European Food Safety Authority (EFSA) (10 mg/day) regulatory limits. These findings provide scientific support for microbial biotransformation and the potential use of anthraquinone compounds, and a way to mitigate the adverse effects (such as intestinal dependence and electrolyte imbalance) of traditional sennoside-based products. This work facilitated the development of C. occidentalis or sennoside-based products for use in functional foods and nutraceuticals and promotes their modernization in herbal applications.

Keywords

Cassia occidentalis L. / sennosides / biotransformation / physcion / intestinal probiotics / Anthraquinone compounds

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