Profile of chemical compounds, aroma, and cup quality of arabica coffee from ohmic-heated carbonic maceration

Abstract

In this study, we investigated the effects of ohmic-heated carbonic maceration on the chemical composition (including bioactive compounds), aroma, and cup quality of Arabica coffee. Coffee cherries were fermented at two temperature levels (31 ℃ and 36 ℃) and three durations (48, 144, and 240 hours) in an ohmic-heated fermenter under carbonic (CO₂-rich, anaerobic) conditions. The coffee samples were analyzed using HPLC and GC-MS to identify bioactive and volatile compounds, and cupping tests were performed using the Specialty Coffee Association of America (SCAA) protocol. The results showed that increasing fermentation duration decreased 3-caffeoylquinic acid (3-CQA) and 4-CQA, while 5-CQA remained stable or increased at 36 ℃ for 240 hours. Trigonelline exhibited relative stability at low temperatures but declined at prolonged durations, particularly at 36 ℃. Caffeine concentrations peaked at intermediate durations before stabilizing. Sugar content generally decreased with increasing fermentation duration, with significantly lower levels observed after 10 days of fermentation. Principal component analysis of volatile compounds revealed distinct clustering patterns among treatments, with long fermentation durations positively correlated with the production of phenols, alcohols, pyrroles, benzenes, pyridines, and alkanes. Sensory evaluation using the SCAA protocol showed that four fermentation treatments provided slightly higher cupping scores (85.0–85.75) than the control (84.5), with flavor notes including nutty, flowery, lemony, brown sugar, spicy, fruity, and winey aromas. These findings indicated that Arabica coffee processed under ohmic-heated carbonic fermentation conditions exhibits measurable modulation of key chemical constituents, sugar content, and volatile composition, leading to improved cup quality and distinct sensory attributes within the studied processing conditions.

Keywords

Arabica coffee / chemical composition / carbonic maceration / ohmic heating / sensory quality

References

[1] Kitzberger CSG, Pot D, Marraccini P, et al. (2020) Flavor precursors and sensory attributes of coffee submitted to different post-harvest processing. AIMS Agric Food 5: 700-714. https://doi.org/10.3934/agrfood.2020.4.700

[2] Velásquez S, Banchón C, Farfán D, et al. (2023) Postharvest effects on the physical quality and sensory characteristics of Coffea canephora. Acta Scientiarum Polonorum Technologia Alimentaria 22: 405-417. https://doi.org/10.17306/J.AFS.2023.1181

[3] Rodriguez YFB, Guzman NG, Hernandez JG (2020) Effect of the postharvest processing method on the biochemical composition and sensory analysis of Arabica coffee. Eng Agríc 40: 177-183. https://doi.org/10.1590/1809-4430-Eng.Agric.v40n2p177-183/2020

[4] Gomes WdS, Pereira LL, Filete CA, et al. (2022) Changes in the chemical and sensory profile of Coffea canephora var. Conilon promoted by carbonic maceration. Agronomy 12: 2265. https://doi.org/10.3390/agronomy12102265

[5] Zakidou P,Plati F,Matsakidou A, et al. (2021) Single origin coffee aroma: From optimized flavor protocols and coffee customization to instrumental volatile characterization and chemometrics. Molecules 26: 4609. https://doi.org/10.3390/molecules26154609

[6] Silva MCS, Veloso TGR, Brioschi Junior D, et al. (2023) Bacterial community and sensory quality from coffee are affected along fermentation under carbonic maceration. Food Chem Adv 3: 100554. https://doi.org/10.1016/j.focha.2023.100554

[7] Silva MES, Oliveira RL, Lucena RM, et al. (2024) Coffee fermentation as a tool for quality improvement: an integrative review and bibliometric analysis. Int J Food Sci Technol 59: 5912-5925. https://doi.org/10.1111/ijfs.17381

[8] Gavahian M, Tiwari BK (2020) Moderate electric fields and ohmic heating as promising fermentation tools. Innovative Food Sci Emerging Technol 64: 102422. https://doi.org/10.1016/j.ifset.2020.102422

[9] Alkanan ZT, Altemimi AB, Al-Hilphy ARS, et al. (2021) Ohmic heating in the food industry: Developments in concepts and applications during 2013-2020. Appl Sci 11: 2507. https://doi.org/10.3390/app11062507

[10] Lalremmawii, Kumar S, Kaur M, et al. (2025) Ohmic heating enhanced extraction and yield kinetics of kinnow juice. Discover Food 5: 206. https://doi.org/10.1007/s44187-025-00499-z

[11] Rojas-González A, Figueroa-Hernández CY, González-Rios O, et al. (2022) Coffee chlorogenic acids incorporation for bioactivity enhancement of foods: A review. Molecules 27: 3400. https://doi.org/10.3390/molecules27113400

[12] Caporaso N, Whitworth MB, Grebby S, et al. (2018) Non-destructive analysis of sucrose, caffeine and trigonelline on single green coffee beans by hyperspectral imaging. Food Res Int 106: 193-203. https://doi.org/10.1016/j.foodres.2017.12.031

[13] de Melo Pereira GV, da Silva Vale A, Ribeiro-Barros AI, et al. (2026) Integrated microbial-metabolomic analysis reveals how fermentation contributes to the unique flavor of African Arabica coffee. Food Chem: Mol Sci 12: 100344. https://doi.org/10.1016/j.fochms.2025.100344

[14] Bernardes PC, Coelho JM, Martins PMM, et al. (2024) Microbial ecology and fermentation of Coffea canephora. Front Food Sci Technol 4: 1377226. https://doi.org/10.3389/frfst.2024.1377226

[15] Balcázar-Zumaeta CR, Castro-Alayo EM, Cayo-Colca IS, et al. (2023) Metabolomics during the spontaneous fermentation in cocoa (Theobroma cacao L.): An exploratory review. Food Res Int 163: 112190. https://doi.org/10.1016/j.foodres.2022.112190

[16] Gavahian M, Tiwari BK (2020) Moderate electric fields and ohmic heating as promising fermentation tools. Innovative Food Sci Emerging Technol 64: 102422. https://doi.org/10.1016/j.ifset.2020.102422

[17] Vaz CJT, de Menezes LS, de Santana RC, et al. (2023) Effect of fermentation on the physicochemical characteristics and sensory quality of Arabica coffee. 3 Biotech 13: 403. https://doi.org/10.1007/s13205-023-03768-9

[18] Reta R, Salengke S, Muhidong J, et al. (2020) Fermentation of Arabica coffee beans using ohmic heating technology in producing specialty coffee. Pelita Perkebunan 36: 226-235. https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v36i3.452

[19] Herawati D, Loisanjaya MO, Kamal RH, et al. (2022) Profile of bioactive compounds, aromas, and cup quality of Excelsa coffee (Coffea liberica var. dewevrei) prepared from diverse postharvest processes. Int J Food Sci 2022: 2365603. https://doi.org/10.1155/2022/2365603

[20] Pandiselvam R, Sathyanath S, Manikantan MR, et al. (2024) Physicochemical properties of coconut inflorescence sap (neera) under double wall open heating system. J Food Meas Charact 18: 8555-8563. https://doi.org/10.1007/s11694-024-02824-z

[21] dos Santos IF, Pimentel TC, da Cruz AG, et al. (2024) Ohmic heating in food processing: An overview of plant-based protein modification. Processes 12: 1800. https://doi.org/10.3390/pr12091800

[22] Fitri N, Nurhaliza N, Anggraeni ES, et al. (2025) Chemical composition, antioxidant activity, and sensory profile of espresso-based Arabica coffee from different bean origins. Beverage Plant Res 5: e008. https://doi.org/10.48130/bpr-0024-0040

[23] Ashihara H (2006) Metabolism of alkaloids in coffee plants. Braz J Plant Physiol 18: 1-8.

[24] Farah A, De Paulis T, Trugo LC, et al. (2005) Effect of roasting on the formation of chlorogenic acid lactones in coffee. J Agric Food Chem 53: 1505-1513. https://doi.org/10.1021/jf048701t

[25] Sagita D, Rukmana J, Utami D, et al. (2026) Effect of green coffee beans fermentation with and without ohmic heating: Physicochemical and sensory properties. J Future Foods 6: 460-469. https://doi.org/10.1016/j.jfutfo.2024.06.001

[26] Shen X, Wang Q, Wang H, et al. (2025) Microbial characteristics and functions in coffee fermentation: A review. Fermentation 11: 5. https://doi.org/10.3390/fermentation11010005

[27] Ky C-L, Louarn J, Dussert S, et al. (2001) Caffeine, trigonelline, chlorogenic acids and sucrose diversity in wild Coffea arabica L. and C. canephora P. accessions. Food Chem 75: 223-230. https://doi.org/10.1016/S0308-8146(01)00204-7

[28] Sarkar S, Sha SP, Ghatani K. (2023) Metabolomics of ethnic fermented foods and beverages: Understanding new aspects through omic techniques. Front Sustainable Food Syst 7: 1040567. https://doi.org/10.3389/fsufs.2023.1040567

[29] Elhalis H, Cox J, Frank D, et al. (2021) Microbiological and chemical characteristics of wet coffee fermentation inoculated with Hansinaspora uvarum and Pichia kudriavzevii and their impact on coffee sensory quality. Front Microbiol 12: 713969. https://doi.org/10.3389/fmicb.2021.713969

[30] Vaz CJT, de Menezes LS, de Santana RC, et al. (2023) Effect of fermentation on the physicochemical characteristics and sensory quality of Arabica coffee. 3 Biotech 13: 403. https://doi.org/10.1007/s13205-023-03768-9

[31] Wang Y, Wang X, Hu G, et al. (2023) Anaerobic germination of green coffee beans: A novel strategy to improve the quality of commercial Arabica coffee. Curr Res Food Sci 6: 100461. https://doi.org/10.1016/j.crfs.2023.100461

[32] Buffo RA, Cardelli-Freire C (2004) Coffee flavour: An overview. Flavour Fragrance J 19: 99-104. https://doi.org/10.1002/ffj.1325

[33] Godillot J, Baconin C, Sanchez I, et al. (2023) Analysis of volatile compounds production kinetics: A study of the impact of nitrogen addition and temperature during alcoholic fermentation. Front Microbiol 14: 1124970. https://doi.org/10.3389/fmicb.2023.1124970

[34] Radeka S, Bestulić E, Rossi S, et al. (2023) Effect of different vinification techniques on the concentration of volatile aroma compounds and sensory profile of Malvazija Istarska wines. Fermentation 9: 676. https://doi.org/10.3390/fermentation9070676

[35] Martínez-Moreno A, Toledo-Gil R, Bautista-Ortin AB, et al. (2024) Exploring the impact of extended maceration on the volatile compounds and sensory profile of Monastrell red wine. Fermentation 10: 343. https://doi.org/10.3390/fermentation10070343

[36] Tarko T, Duda A (2024) Volatilomics of fruit wines. Molecules 29: 2457. https://doi.org/10.3390/molecules29112457

[37] Bianchi A,Pacifico S,Santini G, et al. (2025) Carbonic or nitrogen maceration of wine grape: Biochemical differences of grape and wine using destructive and non-destructive approach. Food Chem 487: 144782. https://doi.org/10.1016/j.foodchem.2025.144782

[38] Zapata J, Londoño V, Naranjo M, et al. (2018) Characterization of aroma compounds present in an industrial recovery concentrate of coffee flavour. CyTA-J Food 16: 367-372. https://doi.org/10.1080/19476337.2017.1406995

[39] Silva LCF,Pereira PVR,Cruz MAD, et al. (2024) Enhancing sensory quality of coffee: The impact of fermentation techniques on Coffea arabica cv. Catiguá MG2. Foods 13: 653. https://doi.org/10.3390/foods13050653

[40] Várady M, Grajzer M, Zalewski I, et al. (2024) Fatty acid composition and sensory properties as descriptors of differentiation of specialty coffees based on spontaneous and induced processing methods. Appl Food Res 4: 100512. https://doi.org/10.1016/j.afres.2024.100512

[41] Brioschi Junior D, Guarçoni RC, Silva MCS, et al. (2021) Microbial fermentation affects sensorial, chemical, and microbial profile of coffee under carbonic maceration. Food Chem 342: 128296. https://doi.org/10.1016/j.foodchem.2020.128296