Physicochemical and sensory functions of rice-based third-generation snacks enriched with chickpea and red pitaya powder

Abstract

The present study evaluated the incorporation of red pitaya powder into a rice/chickpea (80:20) matrix, resulting in four treatments, namely T-2.5 (2.5%), T-5.0 (5.0%), T-7.5 (7.5%), and T-10 (10%) powder addition, which were processed through extrusion cooking, followed by microwave expansion for the development of a third-generation (3G) snack. The physicochemical properties, including expansion index (EI), hardness, and bulk density (BD), as well as functional parameters such as total polyphenol content (TPC) and antioxidant activity (AA), were analyzed. Furthermore, structural modifications generated by processing were examined via X-ray diffraction, FT-IR spectroscopy, and microstructural analyses. The addition of pitaya powder significantly affected the physicochemical properties of the 3G snacks, resulting in a decrease in EI, BD, and hardness after the extrusion and expansion process using microwaves. In addition, the bioactive potential of the snacks improved, generating the highest levels with the addition of pitaya powder, resulting in a 38% increase in TPC and a 3.1-times increase in AA, with a retention of 30% in total betalain content (TBC). Microstructural analyses showed modifications after processing, with a loss of crystalline order and transitions in the type-V diffraction pattern, indicative of amylose–lipid complex formation, characterized by a decrease in the 1047/1022 ratio and stability of 1022/995 ratio. Also, the sensory analysis revealed that the 7.5% formulation was the most attractive, maintaining the principal characteristics of color and texture, with a general acceptance above 38%. These results demonstrate that the incorporation of pitaya powder enhances the bioactive, functional, and sensorial properties of third-generation extruded products.

Keywords

bioactive compounds / chickpea / extrusion / powder red pitaya / sensory evaluation / third-generation snacks

References

[1] Ambroziewicz ZM, Siemiątkowski R, Łata M, et al. (2024) Long-term health effects of artificially colored foods in adults and children. J Educ Health Sport 76: 56522. https://doi.org/10.12775/JEHS.2024.76.56522

[2] Sadighara P, Safta M, Limam I, et al. (2023) Association between food additives and allergic reactions in children: A systematic review. Rev Environ Health 38: 181-186. https://doi.org/10.1515/reveh-2021-0158

[3] Mohidem NA, Hashim N, Shamsudin R, et al. (2022) Rice for food security: Revisiting its production, diversity, rice milling process and nutrient content. Agriculture 12: 741. https://doi.org/10.3390/agriculture12060741

[4] Saeed SMG, Ali SA, Naz J, et al. (2023) Techno-functional, antioxidants, microstructural, and sensory characteristics of biscuits as affected by fat replacer using roasted and germinated chickpea (Cicer arietinum L.). Int J Food Prop 26: 2055-2077. https://doi.org/10.1080/10942912.2023.2242602

[5] Kumar N, Hong S, Zhu Y, et al. (2025) Comprehensive review of chickpea (Cicer arietinum): Nutritional significance, health benefits, techno-functionalities, and food applications. Compr Rev Food Sci F 24: e70152. https://doi.org/10.1111/1541-4337.70152

[6] Hess JM, Jonnalagadda SS, Slavin JL (2016) What is a snack, why do we snack, and how can we choose better snacks? Adv Nutr 7: 466-475. https://doi.org/10.3945/an.115.009571

[7] Mattioli R, Francioso A, Mosca L, et al. (2020) Anthocyanins: Chemical properties and health effects. Molecules 25: 3809. https://doi.org/10.3390/molecules25173809

[8] Khoo HE, Prasad KN, Kong KW, et al. (2011) Carotenoids and their isomers. Molecules 16: 1710-1738. https://doi.org/10.3390/molecules16021710

[9] Vega EN, Mulero MC, Ruiz VF, et al. (2023) Natural sources of food colorants. Foods 12: 4102. https://doi.org/10.3390/foods12224102

[10] Montes ED (2025) Preservation of plant-based pigments via spray drying. Processes 13: 663. https://doi.org/10.3390/pr13030663

[11] Singh S, Aeri V, Sharma V (2023) Encapsulated natural pigments: techniques and applications. J Food Process Eng 46: e14311. https://doi.org/10.1111/jfpe.14311

[12] Acurio L, Salazar D, Segovia PG, et al. (2023) Third-generation snacks from Andean tubers. Foods 12: 2168. https://doi.org/10.3390/foods12112168

[13] Qiu C, Hu H, Chen B, et al. (2024) Research progress on the physicochemical properties of starch-based foods by extrusion processing. Foods 13: 3677. https://doi.org/10.3390/foods13223677

[14] Igual M, Moreau F, Segovia PG, et al. (2023) Valorization of beetroot by-products. Foods 12: 176. https://doi.org/10.3390/foods12010176

[15] Kojić J, Belović M, Krulj J, et al. (2022) Textural, color and sensory features of spelt wholegrain snack enriched with betaine. Foods 11: 475. https://doi.org/10.3390/foods11030475

[16] Kantrong H, Klongdee S, Jantapirak S, et al. (2022) Effects of extrusion temperature and puffing technique on physical and functional properties of purpled third-generation snack after heat treatment. J Food Sci Technol 59: 2209-2219. https://doi.org/10.1007/s13197-021-05234-x

[17] Suárez DN, Pizarro COM, Carrillo EP, et al. (2025) Impact of vegetal protein on microencapsulated pitaya juice. AppliedChem 5: 12. https://doi.org/10.3390/appliedchem5020012

[18] Neder-Suárez D, Quintero-Ramos A, Meléndez-Pizarro CO, et al. (2021) Evaluation of the physicochemical properties of third-generation snacks made from blue corn, black beans, and sweet chard produced by extrusion. LWT 146: 111414. https://doi.org/10.1016/j.lwt.2021.111414

[19] Zambrano Y, Mariotti-Celis MS, Bouchon P (2024) 3G extruded snacks enriched with catechin. LWT 192: 115674. https://doi.org/10.1016/j.lwt.2023.115674

[20] Soto-Dagnino MA, Sánchez-Madrigal MÁ, Heredia-Olea E, et al. (2024) Microencapsulation of pitaya juice (Stenocereus stellatus) by spray drying using mixtures of fructans, whey protein, and modified starch as carrier agents. Biotecnia 26. https://doi.org/10.18633/biotecnia.v26.2268

[21] Pęksa A, Nemś A, Nadal ES, et al. (2025) Sensory profile and consumer acceptability of third generation snacks from colored flesh potatoes. LWT 217: 117460. https://doi.org/10.1016/j.lwt.2025.117460

[22] Altaf U, Hussain SZ, Qadri T, et al. (2020) Optimization of extrusion process for development of nutritious snacks using rice and chickpea flour. J Sci Ind Res 79: 430-436.

[23] Ding QB, Ainsworth P, Plunkett A, et al. (2006) The effect of extrusion conditions on the functional and physical properties of wheat-based expanded snacks. J Food Eng 73: 142-148. https://doi.org/10.1016/j.jfoodeng.2005.01.013

[24] Lisiecka K, Wójtowicz A, Gancarz M (2021) Characteristics of newly developed extruded products supplemented with plants in a form of microwave-rxpanded snacks. Materials 14: 2791. https://doi.org/10.3390/ma14112791

[25] Shah FUH, Sharif MK, Butt MS, et al. (2017) Development of protein, dietary fiber, and micronutrient enriched extruded corn snacks. J Texture Stud 48: 221-230. https://doi.org/10.1111/jtxs.12231

[26] Oyeyinka SA, Akintayo OA, Adebo OA, et al. (2021) A review on the physicochemical properties of starches modified by microwave alone and in combination with other methods. Int J Biol Macromol 176: 87-95. https://doi.org/10.1016/j.ijbiomac.2021.02.066

[27] Lisiecka K, Wójtowicz A (2021) Effect of fresh beetroot application and processing conditions on some quality features of new type of potato-based snacks. LWT 141: 110919. https://doi.org/10.1016/j.lwt.2021.110919

[28] Esteves LC, Pinheiro AC, Pioli RM, et al. (2018) Revisiting the mechanism of hydrolysis of betanin. Photochem Photobiol 94: 853-864. https://doi.org/10.1111/php.12897

[29] Carreón-Hidalgo JP, Franco-Vásquez DC, Gómez-Linton DR, et al. (2022) Betalain plant sources, biosynthesis, extraction, stability enhancement methods, bioactivity, and applications. Food Res Int 151: 110821. https://doi.org/10.1016/j.foodres.2021.110821

[30] Kayın N, Atalay D, Akçay TT, et al. (2019) Color stability and change in bioactive compounds of red beet juice concentrate stored at different temperatures. J Food Sci Technol 56: 5097-5106. https://doi.org/10.1007/s13197-019-03982-5

[31] Žilić S, Mogol BA, Akıllıoğlu G, et al. (2014) Effects of extrusion, infrared and microwave processing on Maillard reaction products and phenolic compounds in soybean. J Sci Food Agr 94: 45-51. https://doi.org/10.1002/jsfa.6210

[32] Pozo C, Rodríguez-Llamazares S, Bouza R, et al. (2018) Study of the structural order of native starch granules using combined FTIR and XRD analysis. J Polym Res 25: 266. https://doi.org/10.1007/s10965-018-1651-y

[33] Yniestra-Marure LM, Núñez-Santiago MC, Agama-Acevedo E, et al. (2019) Starch characterization of improved Chickpea varieties grown in Mexico. Starch-Stärke 71: 1800139. https://doi.org/10.1002/star.201800139

[34] Jia B, Devkota L, Sissons M, et al. (2023) Degradation of starch in pasta induced by extrusion below gelatinization temperature. Food Chem 426: 136524. https://doi.org/10.1016/j.foodchem.2023.136524

[35] Lu H,Ma R,Chang R, et al. (2021) Evaluation of starch retrogradation by infrared spectroscopy. Food Hydrocolloid 120: 106975. https://doi.org/10.1016/j.foodhyd.2021.106975

[36] Liu X, Zhao X, Ma C, et al. (2024) Effects of extrusion technology on physicochemical properties and microstructure of rice starch added with soy protein isolate and whey protein isolate. Foods 13: 764. https://doi.org/10.3390/foods13050764

[37] Yao T, Ma M, Sui Z (2023) Structure and function of polysaccharides and oligosaccharides in foods. Foods 12: 3872. https://doi.org/10.3390/foods12203872

[38] Wu W,Jiao A,Xu E, et al. (2020) Effects of extrusion technology combined with enzymatic hydrolysis on the structural and physicochemical properties of porous corn starch. Food Bioprocess Tech 13: 442-451. https://doi.org/10.1007/s11947-020-02404-1

[39] Wang LS, Duan YM, Tong LF, et al. (2023) Effect of extrusion parameters on the interaction between rice starch and glutelin in the preparation of reconstituted rice. Int J Biol Macromol 225: 277-285. https://doi.org/10.1016/j.ijbiomac.2022.11.009

[40] Minweyelet M, Solomon WK, Bultosa G (2021) Teff-rice extruded blends: physicochemical and sensory properties. Food Res 5: 173-183. https://doi.org/10.26656/fr.2017.5(2).467

[41] Lucas BF, de Morais MG, Santos TD, et al. (2018) Spirulina for snack enrichment: Nutritional, physical and sensory evaluations. LWT 90: 270-276. https://doi.org/10.1016/j.lwt.2017.12.032