Droplet 3D cryobioprinting for fabrication of free-standing and volumetric structures

Joshua Weygant; Ali Entezari; Fritz Koch; Ricardo André Galaviz; Carlos Ezio Garciamendez; Pável Hernández; Vanessa Ortiz; David Sebastián Rendon Ruiz; Francisco Aguilar; Andrea Andolfi; Ling Cai; Sushila Maharjan; Anayancy Osorio; Yu Shrike Zhang

doi:10.1002/agt2.599

Aggregate ›› 2024, Vol. 5 ›› Issue (5) : e599 DOI: 10.1002/agt2.599

RESEARCH ARTICLE

Droplet 3D cryobioprinting for fabrication of free-standing and volumetric structures

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Abstract

Droplet-based bioprinting has shown remarkable potential in tissue engineering and regenerative medicine. However, it requires bioinks with low viscosities, which makes it challenging to create complex 3D structures and spatially pattern them with different materials. This study introduces a novel approach to bioprinting sophisticated volumetric objects by merging droplet-based bioprinting and cryobioprinting techniques. By leveraging the benefits of cryopreservation, we fabricated, for the first time, intricate, self-supporting cell-free or cell-laden structures with single or multiple materials in a simple droplet-based bioprinting process that is facilitated by depositing the droplets onto a cryoplate followed by crosslinking during revival. The feasibility of this approach is demonstrated by bioprinting several cell types, with cell viability increasing to 80%–90% after up to 2 or 3 weeks of culture. Furthermore, the applicational capabilities of this approach are showcased by bioprinting an endothelialized breast cancer model. The results indicate that merging droplet and cryogenic bioprinting complements current droplet-based bioprinting techniques and opens new avenues for the fabrication of volumetric objects with enhanced complexity and functionality, presenting exciting potential for biomedical applications.

Keywords

3D bioprinting / biofabrication / cryogenic bioprinting / droplet printing / inkjet / tissue engineering

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Joshua Weygant, Ali Entezari, Fritz Koch, Ricardo André Galaviz, Carlos Ezio Garciamendez, Pável Hernández, Vanessa Ortiz, David Sebastián Rendon Ruiz, Francisco Aguilar, Andrea Andolfi, Ling Cai, Sushila Maharjan, Anayancy Osorio, Yu Shrike Zhang. Droplet 3D cryobioprinting for fabrication of free-standing and volumetric structures. Aggregate, 2024, 5(5): e599 DOI:10.1002/agt2.599

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