Unlocking 3D printing technology for microalgal production and application

Han Sun , Qian Gong , Yuwei Fan , Yuxin Wang , Jia Wang , Changliang Zhu , Haijin Mou , Shufang Yang , Jin Liu

Advanced Biotechnology ›› 2024, Vol. 2 ›› Issue (4) : 36

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Advanced Biotechnology ›› 2024, Vol. 2 ›› Issue (4) : 36 DOI: 10.1007/s44307-024-00044-6
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Unlocking 3D printing technology for microalgal production and application

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Abstract

Microalgae offer a promising alternative for sustainable nutritional supplements and functional food ingredients and hold potential to meet the growing demand for nutritious and eco-friendly food alternatives. With the escalating impacts of global climate change and increasing human activities, microalgal production must be enhanced by reducing freshwater and land use and minimizing carbon emissions. The advent of 3D printing offers novel opportunities for optimizing microalgae production, though it faces challenges such as high production costs and scalability concerns. This work aims to provide a comprehensive overview of recent advancements in 3D-printed bioreactors for microalgal production, focusing on 3D printing techniques, bio-ink types, and their applications across environmental, food, and medical fields. This review highlights the benefits of 3D-printed bioreactors, including improved mass transfer, optimized light exposure, enhanced biomass yield, and augmented photosynthesis. Current challenges and future directions of 3D printing in microalgal production are also discussed to offer new insights into boosting microalgal cultivation efficiency for expanded applications.

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Han Sun, Qian Gong, Yuwei Fan, Yuxin Wang, Jia Wang, Changliang Zhu, Haijin Mou, Shufang Yang, Jin Liu. Unlocking 3D printing technology for microalgal production and application. Advanced Biotechnology, 2024, 2(4): 36 DOI:10.1007/s44307-024-00044-6

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References

[1]

Alcala-OrozcoCR, MutrejaI, CuiX, HooperGJ, LimKS, WoodfieldTBF. Hybrid biofabrication of 3D osteoconductive constructs comprising Mg-based nanocomposites and cell-laden bioinks for bone repair. Bone, 2022, 154: 116198

[2]

AmorimML, SoaresJ, dos Reis CoimbraJS, LeiteMdO, Teixeira AlbinoLF, MartinsMA. Microalgae proteins: production, separation, isolation, quantification, and application in food and feed. Crit Rev Food Sci, 2021, 61: 1976-2002

[3]

BalasubramanianS, YuK, MeyerAS, KaranaE, Aubin-TamM-E. Bioprinting of Regenerative Photosynthetic Living Materials. Adv Funct Mater, 2021, 31: 2011162

[4]

BranyikovaI, LucakovaSJOA. Technical and physiological aspects of microalgae cultivation and productivity—spirulina as a promising and feasible choice. Org Agr, 2021, 11: 269-276

[5]

CaporgnoMP, MathysA. Trends in Microalgae Incorporation Into Innovative Food Products With Potential Health Benefits. Front Nutr, 2018, 5: 58

[6]

CarlssonAS, Van BileinJB, MöllerR, ClaytonD, BowlesD. Output from EPOBIO Project: Micro- and Macroalgae Utility for Industrial Application, 2007York, U.K.CPL Press1-86

[7]

ChangHK, YangDH, HaMY, KimHJ, KimCH, KimSH, ChoiJW, ChunHJ. 3D printing of cell-laden visible light curable glycol chitosan bioink for bone tissue engineering. Carbohyd Polym, 2022, 287: 119328

[8]

ChoiDJ, ChoiK, ParkSJ, KimY-J, ChungS, KimC-H. Suture Fiber Reinforcement of a 3D Printed Gelatin Scaffold for Its Potential Application in Soft Tissue Engineering. Int J Mol Sci, 2021, 22: 11600

[9]

CrawfordA, In-naP, CaldwellG, ArmstrongR, BridgensB. Clay 3D printing as a bio-design research tool: development of photosynthetic living building components. Archit Sci Rev, 2022, 65: 185-195

[10]

Dang-ThuanT, LeeHR, JungS, ParkMS, YangJ-W. Lipid-extracted algal biomass based biocomposites fabrication with poly (vinyl alcohol). Algal Res, 2018, 31: 525-533

[11]

DattaP, SarkarR, VyasV, BhutoriaS, BaruiA, ChowdhuryA, DattaP. Alginate-honey bioinks with improved cell responses for applications as bioprinted tissue engineered constructs. J Mater Res, 2018, 2018(33): 2029-2039

[12]

DhandwalA, BashirO, MalikT, et al. . Sustainable microalgal biomass as a potential functional food and its applications in food industry: a comprehensive review. Environ Sci Pollut Res, 2024

[13]

DwivediR, MehrotraD. 3D bioprinting and craniofacial regeneration. J Oral Biol Craniofac Res, 2020, 10: 650-659

[14]

FiedlerM, SchoemigO, FischerF, DroederK. Technological Evaluation of Algae-Based Fillers for Polymer 3D Printing. Sustainability, 2023, 15: 4039

[15]

GudapatiH, DeyM, OzbolatI. A comprehensive review on droplet-based bioprinting: Past, present and future. Biomaterials, 2016, 102: 20-42

[16]

HaoL, ZhaoS, HaoS, HeY, FengM, ZhouK, HeY, YangJ, MaoH, GuZ. Functionalized gelatin-alginate based bioink with enhanced manufacturability and biomimicry for accelerating wound healing. Int J Biol Macromol, 2023, 240: 124364

[17]

HeY, DerakhshanfarS, ZhongW, LiB, LuF, XingM, LiX. Characterization and application of carboxymethyl chitosan-based bioink in cartilage tissue engineering. J Nanomater, 2020, 2020: 2057097

[18]

HepburnC, AdlenE, BeddingtonJ, CarterEA, FussS, Mac DowellN, MinxJC, SmithP, WilliamsCK. The technological and economic prospects for CO2 utilization and removal. Nature, 2019, 575: 87-97

[19]

HuangY, ZhangaB, ChenaK, XiaaA, ZhuaX, ZhuaX, LiaoQ. Temperature-controlled microalgae biofilm adsorption/desorption in a thermo-responsive light-guided 3D porous photo-bioreactor for CO2 fixation. Environ Res, 2023, 216: 114645

[20]

ImS, ChoeG, SeokJM, YeoSJ, LeeJH, KimWD, LeeJY, ParkSA. An osteogenic bioink composed of alginate, cellulose nanofibrils, and polydopamine nanoparticles for 3D bioprinting and bone tissue engineering. Int J Biol Macromol, 2022, 205: 520-529

[21]

JohnstonFH, WilliamsonG, Borchers-ArriagadaN, HendersonSB, BowmanDMJS. Climate Change, Landscape Fires, and Human Health: A Global Perspective. Annu Rev Publ Health, 2024, 45: 295-314

[22]

KhoeiniR, et al. . Natural and Synthetic Bioinks for 3D Bioprinting. Adv Nanobiomed Res, 2021, 1: 2000097

[23]

KimYB, LeeH, YangG-H, ChoiCH, LeeD, HwangH, JungW-K, YoonH, KimGH. Mechanically reinforced cell-laden scaffolds formed using alginate-based bioink printed onto the surface of a PCL/alginate mesh structure for regeneration of hard tissue. J Colloid Interf Sci, 2016, 461: 359-368

[24]

KostenkoA, ConnonCJ, SwiokloS. Storable Cell-Laden Alginate Based Bioinks for 3D Biofabrication. Bioengineering, 2023, 10: 23

[25]

KulkarniVR, SahaT, GiriBR, LuAQ, DasSC, ManiruzzamanM. Recent Advancements in Pharmaceutical 3D Printing Industry. J Drug Deliv Sci Tec, 2024, 100: 106072

[26]

LeeJS, SungYJ, SimSJ. Kinetic analysis of microalgae cultivation utilizing 3D-printed real-time monitoring system reveals potential of biological CO2 conversion. Bioresource Technol, 2022, 364: 128014

[27]

LetrasP, OliveiraS, VarelaJ, NunesMC, RaymundoA. 3D printed gluten-free cereal snack with incorporation of Spirulina (Arthrospira platensis) and/or Chlorella vulgaris. Algal Res, 2022, 68: 102863

[28]

LiC, SchrammaN, WangZ, QariNF, JalaalM, LatzMI, CaiS. Ultrasensitive and robust mechanoluminescent living composites. Sci Adv, 2023, 9: eadi8643

[29]

LigonSC, LiskaR, StampflJ, GurrM, MuelhauptR. Polymers for 3D Printing and Customized Additive Manufacturing. Chem Rev, 2017, 117: 10212-10290

[30]

Liu H, et al. Space-Efficient 3D Microalgae Farming with Optimized Resource Utilization for Regenerative Food. Adv Mater. 2024; e2401172-e2401172.

[31]

LodeA, KrujatzF, BrueggemeierS, QuadeM, SchuetzK, KnaackS, WeberJ, BleyT, GelinskyM. Green bioprinting: Fabrication of photosynthetic algae-laden hydrogel scaffolds for biotechnological and medical applications. Eng Life Sci, 2015, 15: 177-183

[32]

MurphySV, AtalaA. 3D bioprinting of tissues and organs. Nat Biotechnol, 2014, 32: 773-785

[33]

NagarajanD, VarjaniS, LeeDJ, ChangJS. Sustainable aquaculture and animal feed from microalgae-Nutritive value and techno-functional components. Renew Sust Energ Rev, 2021, 150: 111549

[34]

NgWL, YeongWY, NaingMW. Polyvinylpyrrolidone-Based Bio-Ink Improves Cell Viability and Homogeneity during Drop-On-Demand Printing. Materials, 2017, 10: 190

[35]

Oh J-J, Ammu S, Vriend VD, Kieffer R, Kleiner FH, Balasubramanian S, Karana E, Masania K, Aubin-Tam M-E. Growth, Distribution, and Photosynthesis of Chlamydomonas Reinhardtii in 3d Hydrogels. Adv Mater. 2023; e2305505-e2305505.

[36]

PahlevanzadehF, MokhtariH, Bakhsheshi-RadHR, EmadiR, KharazihaM, ValianiA, PoursamarSA, IsmailAF, RamaKrishnaS, BertoF. Recent Trends in Three-Dimensional Bioinks Based on Alginate for Biomedical Applications. Materials, 2020, 13: 3980

[37]

PantS, ThomasS, LoganathanS, ValapaRB. 3D bioprinted poly(lactic acid)/mesoporous bioactive glass based biomimetic scaffold with rapid apatite crystallization and in-vitro Cytocompatability for bone tissue engineering. Int J Biol Macromol, 2022, 217: 979-997

[38]

Parra-CabreraC, AchilleC, KuhnS, AmelootR. 3D printing in chemical engineering and catalytic technology: structured catalysts, mixers and reactors. Chem Soc Rev, 2018, 47: 209-230

[39]

PeakCW, SteinJ, GoldKA, GaharwarAK. Nanoengineered colloidal inks for 3D bioprinting. Langmuir, 2018, 34: 917-925

[40]

PilusoS, SkvortsovGA, AltunbekM, AfghahF, KhaniN, KocB, PattersonJ. 3D bioprinting of molecularly engineered PEG-based hydrogels utilizing gelatin fragments. Biofabrication, 2021, 13: 045008

[41]

PozzobonV, OtaolaF, ArnoudtsC, LagirardeJ. Impact of 3D printing materials on mircoalga Chlorella vulgaris. Bioresource Technol, 2023, 389: 129807

[42]

RaeesS, et al. . Classification, processing, and applications of bioink and 3D bioprinting: A detailed review. Int J Biol Macromol, 2023, 232: 123476

[43]

SadeghianmaryanA, NaghiehS, YazdanpanahZ, SardroudHA, SharmaNK, WilsonLD, ChenX. Fabrication of chitosan/alginate/hydroxyapatite hybrid scaffolds using 3D printing and impregnating techniques for potential cartilage regeneration. Int J Biol Macromol, 2022, 204: 62-75

[44]

SathishPB, GayathriS, PriyankaJ, MuthusamyS, NarmadhaR, ShankarKG, SelvakumarR. Tricomposite gelatin-carboxymethylcellulose-alginate bioink for direct and indirect 3D printing of human knee meniscal scaffold. Int J Biol Macromol, 2022, 195: 179-189

[45]

Shahrubudin N, Lee TC, Ramlan R. An Overview on 3D Printing Technology: Technological, Materials, and Applications, 2nd International Conference on Sustainable Materials Processing and Manufacturing (SMPM), South Africa, 2019; pp. 1286–1296.

[46]

ShammaRN, SayedRH, MadryH, El SayedNS, CucchiariniM. Triblock Copolymer Bioinks in Hydrogel Three-Dimensional Printing for Regenerative Medicine: A Focus on Pluronic F127. Tissue Eng Part B-Re, 2022, 28: 451-463

[47]

ShopperlyLK, SpinnenJ, KruegerJ-P, EndresM, SittingerM, LamT, KlokeL, DehneT. Blends of gelatin and hyaluronic acid stratified by stereolithographic bioprinting approximate cartilaginous matrix gradients. J Biomed Mater Res, 2022, 110: 2310-2322

[48]

SomanP, KelberJA, LeeJW, WrightTN, VecchioKS, KlemkeRL, ChenS. Cancer cell migration within 3D layer-by-layer microfabricated photocrosslinked PEG scaffolds with tunable stiffness. Biomaterials, 2012, 33: 7064-7070

[49]

StefanovaA, In-naP, CaldwellGS, BridgensB, ArmstrongR. Photosynthetic textile biocomposites: Using laboratory testing and digital fabrication to develop flexible living building materials. Sci Eng Compos Mater, 2021, 28: 223-236

[50]

SunH, WangY, HeY, LiuB, MouH, ChenF, YangS. Microalgae-Derived Pigments for the Food Industry. Mar Drugs, 2023, 21: 82

[51]

SungYJ, YoonHK, LeeJS, JounJ, YuBS, SirohiR, SimSJ. Novel 3D-printed buoyant structures for improvement in flue gas CO2-derived microalgal biomass production by enhancing anti-biofouling on vertical polymeric photobioreactor. J Clean Prod, 2022, 366: 133030

[52]

SyedMS, RafeieM, HendersonR, VandammeD, AsadniaM, WarkianiME. A 3D-printed mini-hydrocyclone for high throughput particle separation: application to primary harvesting of microalgae. Lab Chip, 2017, 17: 2459-2469

[53]

ThakareK, JerpsethL, PeiZ, TomlinB, QinH. Three-Dimensional Printing of Hydrogel Filters Containing Algae Cells for Copper Removal From Contaminated Water. J Manuf Sci E-T Asme, 2021, 143: 104502

[54]

TianX, JinJ, YuanS, ChuaCK, TorSB, ZhouK. Emerging 3D-Printed Electrochemical Energy Storage Devices: A Critical Review. Adv Energy Mater, 2017, 7: 1700127

[55]

TragerA, NaeimipourS, JuryM, SelegardR, AiliD. Nanocellulose Reinforced Hyaluronan-Based Bioinks. Biomacromol, 2023, 24: 3086-3093

[56]

TripathiS, MandalSS, BauriS, MaitiP. 3D bioprinting and its innovative approach for biomedical applications. Medcomm, 2023, 4: e194

[57]

Uehlin AF. Optimization of a biomimetic poly-(lactic acid) ligament scaffold. 2012; The University of Alabama at Birmingham.

[58]

Uribe-WandurragaZN, ZhangL, NoortMWJ, SchutyserMAI, Garcia-SegoviaP, Martinez-MonzoJ. Printability and Physicochemical Properties of Microalgae-Enriched 3D-Printed Snacks. Food Bioprocess Tech, 2020, 13: 2029-2042

[59]

Uribe-WandurragaZN, IgualM, Reino-MoyonJ, Garcia-SegoviaP, Martinez-MonzoJ. Effect of Microalgae (Arthrospira platensis and Chlorella vulgaris) Addition on 3D Printed Cookies. Food Biophys, 2021, 16: 27-39

[60]

VieiraMV, OliveiraSM, AmadoIR, FasolinLH, VicenteAA, PastranaLM, FucinosP. 3D printed functional cookies fortified with Arthrospira platensis: Evaluation of its antioxidant potential and physical-chemical characterization. Food Hydrocolloid, 2020, 107: 105893

[61]

WangX, YangC, YuY, ZhaoY. In Situ 3D Bioprinting Living Photosynthetic Scaffolds for Autotrophic Wound Healing. Research, 2022, 2022: 9794745

[62]

WangY, YangS, LiuJ, WangJ, XiaoM, LiangQ, RenX, WangY, MouH, SunH. Realization process of microalgal biorefinery: The optional approach toward carbon net-zero emission. Sci Total Environ, 2023, 901: 165546-165546

[63]

WangJ, WangY, GuZ, MouH, SunH. Stimulating carbon and nitrogen metabolism of Chlorella pyrenoidosa to treat aquaculture wastewater and produce high-quality protein in plate photobioreactors. Sci Total Environ, 2023, 878: 163061

[64]

WangJ, WangY, XiaoM, LiangQ, YangS, LiuJ, ZhangY, MouH, SunH. Upcycling food waste into biorefinery production by microalgae. Chem Eng J, 2024, 484: 149532

[65]

WangY, WangJ, YangS, LiangQ, GuZ, WangY, MouH, SunH. Selecting a preculture strategy for improving biomass and astaxanthin productivity of Chromochloris zofingiensis. Appl Microbiol Biot, 2024, 108: 18-18

[66]

Wang H, Bi S, Shi B, Ma J, Lv X, Qiu J, Wei Y. Recent Advances in Engineering Bioinks for 3D Bioprinting. Adv Eng Mater. 2023a; 25.

[67]

WangpraseurtD, et al. . Bionic 3D printed corals. Nat Commun, 2020, 11: 1748

[68]

WangpraseurtD, YouS, SunY, ChenS. Biomimetic 3D living materials powered by microorganisms. Trends Biotechnol, 2022, 40: 843-857

[69]

WuX, ChenK, ChaiQ, LiuS, FengC, XuL, ZhangD. Freestanding vascular scaffolds engineered by direct 3D printing with Gt-Alg-MMT bioinks. Biomater Adv, 2022, 133: 112658-112658

[70]

YangY, WangZ, XuY, XiaJ, XuZ, ZhuS, JinM. Preparation of Chitosan/Recombinant Human Collagen-Based Photo-Responsive Bioinks for 3D Bioprinting. Gels, 2022, 8: 314

[71]

YangS, WangY, WangJ, ChengK, LiuJ, HeY, ZhangY, MouH, SunH. Microalgal protein for sustainable and nutritious foods: A joint analysis of environmental impacts, health benefits and consumer’s acceptance. Trends Food Sci Tech, 2024, 143: 104278

[72]

Yang S, Fan Y, Cao Y, Wang Y, Mou H, Sun H. Technological readiness of commercial microalgae species for foods. Crit Rev Food Sci. 2023; 1–25.

[73]

YarnoldJ, KaranH, OeyM, HankamerB. Microalgal Aquafeeds As Part of a Circular Bioeconomy. Trends Plant Sci, 2019, 24: 959-970

[74]

YuZ, ZhaoW, SunH, MouH, LiuJ, YuH, DaiL, KongQ, YangS. Phycocyanin from microalgae: A comprehensive review covering microalgal culture, phycocyanin sources and stability. Food Res Int, 2024, 186: 114362

[75]

ZengW, ChenK, HuangY, XiaA, ZhuX, ZhuX, LiaoQ. Three-dimensional porous biofilm photobioreactor with light-conducting frameworks for high-efficiency microalgal growth. Algal Res, 2023, 69: 102942

[76]

ZhaoL, ZengG, GuY, TangZ, WangG, TangT, ShanY, SunY. Nature inspired fractal tree-like photobioreactor via 3D printing for CO2 capture by microaglae. Chem Eng Sci, 2019, 193: 6-14

[77]

ZhaoS, GuoC, KumarasenaA, OmenettoFG, KaplanDL. 3D Printing of Functional Microalgal Silk Structures for Environmental Applications. ACS Biomater Sci Eng, 2019, 5: 4808-4816

[78]

ZhengZ, WuJ, LiuM, WangH, LiC, RodriguezMJ, LiG, WangX, KaplanDL. 3D Bioprinting of Self-Standing Silk-Based Bioink. Adv Healthc Mater, 2018, 7: 1701026

[79]

ZhuW, MaX, GouM, MeiD, ZhangK, ChenS. 3D printing of functional biomaterials for tissue engineering. Curr Opin Biotech, 2016, 40: 103-112

[80]

ZouS, FanS, OliveiraAL, YaoX, ZhangY, ShaoH. 3D Printed Gelatin Scaffold with Improved Shape Fidelity and Cytocompatibility by Using Antheraea pernyi Silk Fibroin Nanofibers. Adv Fiber Mater, 2022, 4: 758-773

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