Advances in biotechnology and breeding innovations in China’s marine aquaculture

Abstract

Biotechnology is the key driving force behind the sustainable development of aquaculture, as biological innovation would significantly improve the capabilities of aquatic breeding and achieve independent and controllable seeding sources to ensure food safety. In this article, we have analyzed the current status and existing problems of marine aquaculture in China. Based on these data, we have summarized the recent (especially the last 10 years) biotechnological innovation and breeding progress of marine aquaculture in China, including whole genome sequencing, sex-related marker screening, genomic selection, and genome editing, as well as progress of improved marine fish varieties in China. Finally, the perspectives in this field have been discussed, and three future countermeasures have been proposed.

References

[1] AoJ, MuY, XiangLX, FanD, FengM, ZhangS, ShiQ, ZhuLY, LiT, DingY, NieL, LiQ, DongWR, JiangL, SunB, ZhangX, LiM, ZhangHQ, XieS, ZhuY, JiangX, WangX, MuP, ChenW, YueZ, WangZ, WangJ, ShaoJZ, ChenX. Genome sequencing of the perciform fish Larimichthys crocea provides insights into molecular and genetic mechanisms of stress adaptation. PLoS Genet., 2015, 11(4): e1005118

[2] BaiCM, XinLS, RosaniU, WuB, WangQC, DuanXK, LiuZH, WangCM. Chromosomal level assembly of the blood clam, Scapharca (Anadara) broughtonii, using long sequence reads and Hi-C. Gigascience, 2019, 8(7): giz067

[3] BaiY, WangJ, ZhaoJ, KeQ, QuA, DengY, ZengJ, GongJ, ChenJ, PanY, ChiH, GongH, ZhouT, XuP. Genomic selection for visceral white-nodules diseases resistance in large yellow croaker. Aquaculture., 2022, 559: 738421

[4] BaoY, ZengQ, WangJ, ZhangZ, ZhangY, WangS, WongNK, YuanW, HuangY, ZhangW, LiuJ, LvL, XueQ, ZhaS, PengZ, YaoH, BaoZ, WangS, LinZ. Genomic Insights into the Origin and Evolution of Molluscan Red-Bloodedness in the Blood Clam Tegillarca granosa. Mol Biol Evol., 2021, 38(6): 2351-2365

[5] BianL, LiF, GeJ, WangP, ChangQ, ZhangS, LiJ, LiuC, LiuK, LiuX, LiX, ChenH, ChenS, ShaoC, LinZ. Chromosome-level genome assembly of the greenfin horse-faced filefish (Thamnaconus septentrionalis) using Oxford Nanopore PromethION sequencing and Hi-C technology. Mol Ecol Resour., 2020, 20(4): 1069-1079

[6] Bureau of Fisheries, National Fisheries Technology Extension Center, China Society of Fisheries. China Fishery Statistical Yearbook, 2024Beijing, ChinaChina Agriculture Press

[7] CaiM, ZouY, XiaoS, LiW, HanZ, HanF, XiaoJ, LiuF, WangZ. Chromosome assembly of Collichthys lucidus, a fish of Sciaenidae with a multiple sex chromosome system. Sci Data., 2019, 6(1): 132

[8] Cao X, Zhang J, Deng S, Ding S. Chromosome-Level Genome Assembly of the Speckled Blue Grouper (Epinephelus cyanopodus) Provides Insight into Its Adaptive Evolution. Biology (Basel). 2022;11(12). https://doi.org/10.3390/biology11121810.

[9] Chen B, Zhou Z, Ke Q, Wu Y, Bai H, Pu F, Xu P. The sequencing and de novo assembly of the Larimichthys crocea genome using PacBio and Hi-C technologies. Sci Data. 2019;6(1). https://doi.org/10.1038/s41597-019-0194-3.

[10] ChenL, LuY, LiW, RenY, YuM, JiangS, FuY, WangJ, PengS, BilykKT, MurphyKR, ZhuangX, HuneM, ZhaiW, WangW, XuQ, ChengCHC. The genomic basis for colonizing the freezing Southern Ocean revealed by Antarctic toothfish and Patagonian robalo genomes. Gigascience., 2019, 8(4): giz016

[11] ChenL, ZhouZ, ZhouZ, YangJ, DengY, BaiY, PuF, ZhouT, XuP. Chromosome-level assembly and gene annotation of Decapterus maruadsi genome using Nanopore and Hi-C technologies. Sci Data., 2024, 11(1): 69

[12] ChenSL, JiXS, ShaoCW, LiWL, YangJF, LiangZ, LiaoXL, XuGB, XuY, SongWT. Induction of mitogynogenetic diploids and identification of WW super-female using sex-specific SSR markers in half-smooth tongue sole (Cynoglossus semilaevis). Mar Biotechnol (NY), 2012, 14(1): 120-128

[13] ChenSL, LiJ, DengSP, TianYS, WangQY, ZhuangZM, ShaZX, XuJY. Isolation of female-specific AFLP markers and molecular identification of genetic sex in half-smooth tongue sole (Cynoglossus semilaevis). Mar Biotechnol, 2007, 9(2): 273-280

[14] ChenS, ZhangG, ShaoC, HuangQ, LiuG, ZhangP, SongW, AnN, ChalopinD, VolffJN, HongY, LiQ, ShaZ, ZhouH, XieM, YuQ, LiuY, XiangH, WangN, WuK, YangC, ZhouQ, LiaoX, YangL, HuQ, ZhangJ, MengL, JinL, TianY, LianJ, YangJ, MiaoG, LiuS, LiangZ, YanF, LiY, SunB, ZhangH, ZhangJ, ZhuY, DuM, ZhaoY, SchartlM, TangQ, WangJ. Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle. Nat Genet., 2014, 46(3): 253-260

[15] ChenT, RenC, WongNK, YanA, SunC, FanD, LuoP, JiangX, ZhangL, RuanY, LiJ, WuX, HuoD, HuangJ, LiX, WuFEZ, ChengC, ZhangX, WangY, HuC. The Holothuria leucospilota genome elucidates sacrificial organ expulsion and bioadhesive trap enriched with amyloid-patterned proteins. Proc Natl Acad Sci U S A, 2023, 120(16): 2213512120

[16] CorreaK, LhorenteJP, LópezME, BassiniL, NaswaS, DeebN, Di GenovaA, MaassA, DavidsonWS, YáñezJM. Genome-wide association analysis reveals loci associated with resistance against Piscirickettsia salmonis in two Atlantic salmon (Salmo salar L.) chromosomes. Bmc Genomics., 2015, 16(1): 854

[17] Cui Z K, Liu Y, Wang W W, Wang Q, Zhang N, Lin F, Wang N, Shao C W, Dong Z D, Li Y Z, Yang Y M, Hu M Z, Li H L, Gao F T, Wei Z F, Meng L, Liu Y, Wei M, Zhu Y, Guo H, Cheng C H K, Schartl M, Chen S L. Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis). Sci Rep-Uk. 2017;7. https://doi.org/10.1038/srep42213.

[18] CuiZ, ZhangJ, SunZ, LiuB, ZhaoC, ChangY. Identification of sex-specific markers through 2b-RAD sequencing in the sea urchin (Mesocentrotus nudus). Front Genet, 2021, 12: 717538

[19] DaiP, KongJ, LiuJ, LuX, SuiJ, MengX, LuanS. Evaluation of the utility of genomic information to improve genetic evaluation of feed efficiency traits of the Pacific white shrimp Litopenaeus vannamei. Aquaculture., 2020, 527: 735421

[20] DingJ, ZhangY, LiX, WangJ, GaoX, XiangQ, GaoZ, LanT, JiaS, LuM, MengR, WangX, WuX, ZhuJ, ShenW. Genomic selection for hypoxia tolerance in large yellow croaker. Aquaculture., 2024, 579: 740212

[21] DongL, XiaoS, ChenJ, WanL, WangZ. Genomic Selection Using Extreme Phenotypes and Pre-Selection of SNPs in Large Yellow Croaker (Larimichthys crocea). Mar Biotechnol, 2016, 18(5): 575-583

[22] DongL, XiaoS, WangQ, WangZ. Comparative analysis of the GBLUP, emBayesB, and GWAS algorithms to predict genetic values in large yellow croaker (Larimichthys crocea). BMC Genomics, 2016, 17(1): 460

[23] Dong Y, Zeng Q, Ren J, Yao H, Lv L, He L, Ruan W, Xue Q, Bao Z, Wang S, Lin Z. The Chromosome-Level Genome Assembly and Comprehensive Transcriptomes of the Razor Clam (Sinonovacula constricta). Front Genet,2020;11. https://doi.org/10.3389/fgene.2020.00664.

[24] DongZD, XuWT, ZhangN, LiuY, ShaoCW, WenHS, ChenSL. A rapid method for genetic sexuality identification in Cynoglossus semilaevis. J Agr Biotechnol., 2016, 24(5): 764-769 in Chinese)

[25] DouJ, LiX, FuQ, JiaoW, LiY, LiT, WangY, HuX, WangS, BaoZ. Evaluation of the 2b-RAD method for genomic selection in scallop breeding. Sci Rep-Uk., 2016, 6(1): 19244

[26] Gan H M, Tan M H, Austin C M, Sherman C D H, Wong Y T, Strugnell J, Gervis M, McPherson L, Miller A D. Best Foot Forward: Nanopore Long Reads, Hybrid Meta-Assembly, and Haplotig Purging Optimizes the First Genome Assembly for the Southern Hemisphere Blacklip Abalone (Haliotis rubra). Front Genet. 2019;10. https://doi.org/10.3389/fgene.2019.00889.

[27] GaoY, GaoQ, ZhangH, WangL, ZhangF, YangC, SongL. Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus. DNA Res., 2014, 21(6): 627-637

[28] GeH, LinK, ShenM, WuS, WangY, ZhangZ, WangZ, ZhangY, HuangZ, ZhouC, LinQ, WuJ, LiuL, HuJ, HuangZ, ZhengL. De novo assembly of a chromosome-level reference genome of red-spotted grouper (Epinephelus akaara) using nanopore sequencing and Hi-C. Mol Ecol Resour., 2019, 19(6): 1461-1469

[29] GongJ, ZhaoJ, KeQ, LiB, ZhouZ, WangJ, ZhouT, ZhengW, XuP. First genomic prediction and genome-wide association for complex growth-related traits in Rock Bream (Oplegnathus fasciatus). Evol Appl, 2021, 15(4): 523-536

[30] Gui T S, Zhang J Q, Song F G, Sun Y Y, Xie S J, Yu K J, Xiang J H. CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda. G3-Genes Genom Genet. 2016;6(11):3757–3764. https://doi.org/10.1534/g3.116.034082.

[31] GuoL, YangJW, LiuBS, ZhangN, ZhuKC, GuoHY, MaQW, LiYI, JiangSG, ZhangDC. Colinearity based sex-specific marker development in the golden pompano (Trachinotus ovatus). Aquaculture., 2021, 544: 737044

[32] GuoZ, YangQ, HuangF, ZhengH, SangZ, XuY, ZhangC, WuK, TaoJ, PrasannaBM, OlsenMS, WangY, ZhangJ, XuY. Development of high-resolution multiple-SNP arrays for genetic analyses and molecular breeding through genotyping by target sequencing and liquid chip. Plant Communications., 2021, 2(6): 100230

[33] GutierrezAP, MatikaO, BeanTP, HoustonRD. Genomic Selection for Growth Traits in Pacific Oyster (Crassostrea gigas): Potential of Low-Density Marker Panels for Breeding Value Prediction. Front Genet, 2018, 9: 391

[34] HanW, LiuL, WangJ, WeiH, LiY, ZhangL, GuoZ, LiY, LiuT, ZengQ, XingQ, ShuY, WangT, YangY, ZhangM, LiR, YuJ, PuZ, LvJ, LianS, HuJ, HuX, BaoZ, BaoL, ZhangL, WangS. Ancient homomorphy of molluscan sex chromosomes sustained by reversible sex-biased genes and sex determiner translocation. Nat Ecol Evol., 2022, 6(12): 1891-1906

[35] HanW, WuS, DingH, WangM, WangM, BaoZ, WangB, HuJ. Improved chromosomal-level genome assembly and re-annotation of leopard coral grouper. Sci Data., 2023, 10(1): 156

[36] HeY, ChangY, BaoL, YuM, LiR, NiuJ, FanG, SongW, SeimI, QinY, LiX, LiuJ, KongX, PengM, SunM, WangM, QuJ, WangX, LiuX, WuX, ZhaoX, WangX, ZhangY, GuoJ, LiuY, LiuK, WangY, ZhangH, LiuL, WangM, YuH, WangX, ChengJ, WangZ, XuX, WangJ, YangH, LeeSM, LiuX, ZhangQ, QiJ. A chromosome-level genome of black rockfish, Sebastes schlegelii, provides insights into the evolution of live birth. Mol Ecol Resour, 2019, 19(5): 1309-1321

[37] Higuchi K, Kazeto Y, Ozaki Y, Yamaguchi T, Shimada Y, Ina Y, Soma S, Sakakura Y, Goto R, Matsubara T, Nishiki I, Iwasaki Y, Yasuike M, Nakamura Y, Matsuura A, Masuma S, Sakuma T, Yamamoto T, Masaoka T, Kobayashi T, Fujiwara A, Gen K. Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis). Sci Rep-Uk. 2019;9. https://doi.org/10.1038/s41598-019-50418-3.

[38] HuG, GuW, ChengL, WangB. Genomic prediction and variance component estimation for carcass fat content in rainbow trout using SNP markers. J World Aquacult Soc., 2019, 51(2): 501-511

[39] HuanP, CuiM, WangQ, LiuB. CRISPR/Cas9-mediated mutagenesis reveals the roles of calaxin in gastropod larval cilia. Gene., 2021, 787: 145640

[40] IrizarryRA. Summaries of Affymetrix GeneChip probe level data. Nucleic Acids Res., 2003, 31(4): 15e-15

[41] KangZ, KongJ, SuiJ, DaiP, LuoK, MengX, LiuJ, ChenB, CaoJ, TanJ, FuQ, LiQ, LuanS. Optimal open nucleus breeding system for long-term genetic gain in the Pacific white shrimp using genomic selection. Aquaculture., 2024, 586: 740760

[42] Kawamura W, Hasegawa N, Yamauchi A, Kimura T, Yahagi H, Tani R, Morita T, Yazawa R, Yoshizaki G. Production of albino chub mackerel (Scomber japonicus) by slc45a2 knockout and the use of a positive phototaxis-based larviculture technique to overcome the lethal albino phenotype. Aquaculture. 2022;560. https://doi.org/10.1016/j.aquaculture.2022.738490.

[43] KeQ, WangJ, BaiY, ZhaoJ, GongJ, DengY, QuA, SuoN, ChenJ, ZhouT, XuP. GWAS and genomic prediction revealed potential for genetic improvement of large yellow croaker adapting to high plant protein diet. Aquaculture., 2022, 553: 738090

[44] Kim J, Cho J Y, Kim J W, Kim D G, Nam B H, Kim B S, Kim W J, Kim Y O, Cheong J, Kong H J. Molecular Characterization of Paralichthys olivaceus MAF1 and Its Potential Role as an Anti-Viral Hemorrhagic Septicaemia Virus Factor in Hirame Natural Embryo Cells. Int J Mol Sci. 2021;22(3). https://doi.org/10.3390/ijms22031353.

[45] Kim J, Cho J Y, Kim J W, Kim H C, Noh J K, Kim Y O, Hwang H K, Kim W J, Yeo S Y, An C M, Park J Y, Kong H J. CRISPR/Cas9-mediated myostatin disruption enhances muscle mass in the olive flounder Paralichthys olivaceus. Aquaculture. 2019;512. https://doi.org/10.1016/j.aquaculture.2019.734336.

[46] Li A, Dai H, Guo X, Zhang Z, Zhang K, Wang C, Wang X, Wang W, Chen H, Li X, Zheng H, Li L, Zhang G. Genome of the estuarine oyster provides insights into climate impact and adaptive plasticity. Commun Biol. 2021a;4(1). https://doi.org/10.1038/s42003-021-02823-6.

[47] LiC, LiuX, LiuB, MaB, LiuF, LiuG, ShiQ, WangC. Draft genome of the Peruvian scallop Argopecten purpuratus. Gigascience., 2018, 7(4): giy031

[48] LiF, BianL, GeJ, HanF, LiuZ, LiX, LiuY, LinZ, ShiH, LiuC, ChangQ, LuB, ZhangS, HuJ, XuD, ShaoC, ChenS. Chromosome-level genome assembly of the East Asian common octopus (Octopus sinensis) using PacBio sequencing and Hi-C technology. Mol Ecol Resour., 2020, 20(6): 1572-1582

[49] LiM, XuH, XuWT, ZhouQ, XuXW, ZhuY, ZhengWW, LiWS, PangZF, ChenSL. Isolation of a Male-Specific Molecular Marker and Development of a Genetic Sex Identification Technique in Spotted Knifejaw (Oplegnathus punctatus). Mar Biotechnol, 2020, 22(4): 467-474

[50] LiM, XuXW, LiuSS, FanGY, ZhouQ, ChenSL. The chromosome-level genome assembly of the Japanese yellowtail jack Seriola aureovittata provides insights into genome evolution and efficient oxygen transport. Mol Ecol Resour., 2022, 22(7): 2701-2712

[51] LiM, ZhangR, FanG, XuW, ZhouQ, WangL, LiW, PangZ, YuM, LiuQ, LiuX, SchartlM, ChenS. Reconstruction of the Origin of a Neo-Y Sex Chromosome and Its Evolution in the Spotted Knifejaw, Oplegnathus punctatus. Mol Biol Evol., 2021, 38(6): 2615-2626

[52] LiS, XuL, ShiY, ChenJ. Male-specific markers developed by next-generation sequencing confirmed an XX/XY sex-determination system in farmed ayu (Plecoglossus altivelis). Aquaculture., 2021, 541: 736822

[53] Li X, Qi J, Corush J B, Chen J, Zhang J. A chromosome-level genome assembly of the Walking goby (Scartelaos histophorus). Front Mar Sci. 2022;9. https://doi.org/10.3389/fmars.2022.966275.

[54] Li Y, Sun X, Hu X, Xun X, Zhang J, Guo X, Jiao W, Zhang L, Liu W, Wang J, Li J, Sun Y, Miao Y, Zhang X, Cheng T, Xu G, Fu X, Wang Y, Yu X, Huang X, Lu W, Lv J, Mu C, Wang D, Li X, Xia Y, Li Y, Yang Z, Wang F, Zhang L, Xing Q, Dou H, Ning X, Dou J, Li Y, Kong D, Liu Y, Jiang Z, Li R, Wang S, Bao Z. Scallop genome reveals molecular adaptations to semi-sessile life and neurotoxins. Nat Commun. 2017;8(1). https://doi.org/10.1038/s41467-017-01927-0.

[55] LiY, WangR, XunX, WangJ, BaoL, ThimmappaR, DingJ, JiangJ, ZhangL, LiT, LvJ, MuC, HuX, ZhangL, LiuJ, LiY, YaoL, JiaoW, WangY, LianS, ZhaoZ, ZhanY, HuangX, LiaoH, WangJ, SunH, MiX, XiaY, XingQ, LuW, OsbournA, ZhouZ, ChangY, BaoZ, WangS. Sea cucumber genome provides insights into saponin biosynthesis and aestivation regulation. Cell Discov, 2018, 4: 29

[56] LillehammerM, BangeraR, SalazarM, VelaS, ErazoEC, SuarezA, CockJ, RyeM, RobinsonNA. Genomic selection for white spot syndrome virus resistance in whiteleg shrimp boosts survival under an experimental challenge test. Sci Rep-Uk., 2020, 10(1): 20571

[57] LinA, XiaoS, XuS, YeK, LinX, SunS, WangZ. Identification of a male-specific DNA marker in the large yellow croaker (Larimichthys crocea). Aquaculture, 2017, 480: 116-122

[58] LinQ, FanS, ZhangY, XuM, ZhangH, YangY, LeeAP, WolteringJM, RaviV, GunterHM, LuoW, GaoZ, LimZW, QinG, SchneiderRF, WangX, XiongP, LiG, WangK, MinJ, ZhangC, QiuY, BaiJ, HeW, BianC, ZhangX, ShanD, QuH, SunY, GaoQ, HuangL, ShiQ, MeyerA, VenkateshB. The seahorse genome and the evolution of its specialized morphology. Nature., 2016, 540(7633): 395-399

[59] LinQ, QiuY, GuR, XuM, LiJ, BianC, ZhangH, QinG, ZhangY, LuoW, ChenJ, YouX, FanM, SunM, XuP, VenkateshB, XuJ, FuH, ShiQ. Draft genome of the lined seahorse, Hippocampus erectus. Gigascience., 2017, 6(6): gix030

[60] LinW, XiaoQ, YuF, HanZ, LiuJ, PengW, HuangZ, LeiY, LiW, YouW, LuoX, KeC. Development of a low-density SNP genotyping panel by a novel technology mGPS and its application in germplasm identification of abalone. Aquaculture., 2023, 565: 739089

[61] Lin X Y, Xiao S J, Li W B, Wang Z Y. Development and validation of sex-specific SNP markers in Larimichthys crocea. J Fish China. 2018;42(9):1329–1337. https://doi.org/10.11964/jfc.20180211182. (in Chinese).

[62] LiuG, DongL, GuL, HanZ, ZhangW, FangM, WangZ. Evaluation of Genomic Selection for Seven Economic Traits in Yellow Drum (Nibea albiflora). Mar Biotechnol, 2019, 21(6): 806-812

[63] LiuJ, PengW, YuF, LinW, ShenY, YuW, GongS, HuangH, YouW, LuoX, KeC. Development and validation of a 40-K multiple-SNP array for Pacific abalone (Haliotis discus hannai). Aquaculture, 2022, 558

[64] LiuJ, YinZ, ZhouM, YuW, YouW, ChenY, LuoX, KeC. Genetic parameters and genomic prediction for nutritional quality-related traits of Pacific abalone (Haliotis discus hannai). Aquaculture., 2024, 579: 740118

[65] LiuM, DaiP, KongJ, MengX, SuiJ, LuoK, ChenB, FuQ, CaoB, CaoJ, LuanS. Assessing accuracy of genomic breeding values of selection candidates under biosecurity restrictions by progeny testing in Chinese shrimp Fenneropenaeus chinensis. Aquaculture., 2023, 566: 739181

[66] Liu M, Li X, Kong J, Meng X, Chen M, Luo K, Sui J, Dai P, Zhang Y, Qiang G, Tan J, Chen B, Cao J, Li B, Lai X, Luan S. Application of the liquid chip "Yellow Sea Chip No.1" in genetic evaluation of the base population with resistance to acute hepatopancreatic necrosis disease in Litopenaeus vannamei. J Fish China. 2023;47(1):019417. https://doi.org/10.11964/jfc.20211013127. (in Chinese).

[67] LiuP, LvJ, MaC, ZhangT, HuangX, YangZ, ZhangL, HuJ, WangS, BaoZ. Targeted Genotyping of a Whole-Gene Repertoire by an Ultrahigh-Multiplex and Flexible HD-Marker Approach. Engineering-Prc, 2022, 13: 186-196

[68] Liu X, Li C, Chen M, Liu B, Yan X, Ning J, Ma B, Liu G, Zhong Z, Jia Y, Shi Q, Wang C. Draft genomes of two Atlantic bay scallop subspecies Argopecten irradians irradians and A. i. concentricus. Sci Data. 2020;7(1). https://doi.org/10.1038/s41597-020-0441-7.

[69] Liu Y, Chen S L, Gao F T, Meng L, Hu Q M, Song W T, Shao C W, Lv W Q. SCAR-transformation of sex-specific SSR marker and its application in half-smooth tongue sole(Cynoglossus semiliaevis). J Agr Biotechnol, 2014; 22(6):787–792. https://doi.org/10.3969/j.issn.1674-7968.2014.06.015. (in Chinese).

[70] LiuY, LuS, LiuF, ShaoC, ZhouQ, WangN, LiY, YangY, ZhangY, SunH, ZhengW, ChenS. Genomic Selection Using BayesCπ and GBLUP for Resistance Against Edwardsiella tarda in Japanese Flounder (Paralichthys olivaceus). Mar Biotechnol, 2018, 20(5): 559-565

[71] Lu S, Li Y Z, Wang L, Liu Y, Cheng X M, Zhou Q, Yang Y M, Zheng W W, Chen S L. Application of genomic selection in the breeding of new variety of Chinese tongue sole (Cynoglossus semilaevis) “Tayou No.1”. J Fish China. 2022;46(8):1305–1312. https://doi.org/10.11964/jfc.20220313391. (in Chinese).

[72] LuS, LiuY, QuS, ZhouQ, WangL, ZhangT, XuW, ZhangM, SongY, WangJ, ZhuC, ChenS. Genomic prediction of survival against Vibrio harveyi in leopard coral grouper (Plectropomus leopardus) using GBLUP, weighted GBLUP, and BayesCπ. Aquaculture., 2023, 572: 739536

[73] LuS, LiuY, YuX, LiY, YangY, WeiM, ZhouQ, WangJ, ZhangY, ZhengW, ChenS. Prediction of genomic breeding values based on pre-selected SNPs using ssGBLUP, WssGBLUP and BayesB for Edwardsiellosis resistance in Japanese flounder. Genet Sel Evol., 2020, 52(1): 49

[74] LuS, ZhouQ, ChenY, LiuY, LiY, WangL, YangY, ChenS. Development of a 38 K single nucleotide polymorphism array and application in genomic selection for resistance against Vibrio harveyi in Chinese tongue sole. Cynoglossus semilaevis. Genomics., 2021, 113(4): 1838-1844

[75] LüZ, GongL, RenY, ChenY, WangZ, LiuL, LiH, ChenX, LiZ, LuoH, JiangH, ZengY, WangY, WangK, ZhangC, JiangH, WanW, QinY, ZhangJ, ZhuL, ShiW, HeS, MaoB, WangW, KongX, LiY. Large-scale sequencing of flatfish genomes provides insights into the polyphyletic origin of their specialized body plan. Nat Genet., 2021, 53(5): 742-751

[76] Luo H, Zhang Y, Liu F, Zhao Y, Peng J, Xu Y, Chen X, Huang Y, Ji C, Liu Q, He P, Feng P, Yang C, Wei P, Ma Z, Qin J, Zhou S, Dai S, Zhang Y, Zhao Z, Liu H, Zheng H, Zhang J, Lin Y, Chen X. The male and female genomes of golden pompano (Trachinotus ovatus) provide insights into the sex chromosome evolution and rapid growth. J Adv Res. 2023. https://doi.org/10.1016/j.jare.2023.11.030.

[77] LuoZ, YuY, BaoZ, LiF. Evaluation of machine learning method in genomic selection for growth traits of Pacific white shrimp. Aquaculture., 2024, 581: 740376

[78] LvJ, JiaoW, GuoH, LiuP, WangR, ZhangL, ZengQ, HuX, BaoZ, WangS. HD-Marker: a highly multiplexed and flexible approach for targeted genotyping of more than 10,000 genes in a single-tube assay. Genome Res., 2018, 28(12): 1919-1930

[79] LvJ, LiR, SuZ, GaoB, TiX, YanD, LiuG, LiuP, WangC, LiJ. A chromosome-level genome of Portunus trituberculatus provides insights into its evolution, salinity adaptation and sex determination. Mol Ecol Resour., 2022, 22(4): 1606-1625

[80] LvJ, SunD, HuanP, SongL, LiuP, LiJ. QTL mapping and marker identification for sex-determining: indicating XY sex determination system in the swimming crab (Portunus trituberculatus). Front Genet., 2018, 9: 337

[81] MaHY, ChenSL, YangJF, JiXS, ChenSQ, TianYS, BiJZ. Isolation of sex-specific AFLP markers in Spotted Halibut (Verasper variegatus). Environ Biol Fish, 2010, 88(1): 9-14

[82] MankJE. Sex chromosomes and the evolution of sexual dimorphism: lessons from the genome. Am Nat, 2009, 173(2): 141-150

[83] ØdegårdJ, MoenT, SantiN, KorsvollSA, KjøglumS, MeuwissenTHE. Genomic prediction in an admixed population of Atlantic salmon (Salmo salar). Front Genet, 2014, 5: 402

[84] Ohama M, Washio Y, Kishimoto K, Kinoshita M, Kato K. Growth performance of myostatin knockout red sea bream Pagrus major juveniles produced by genome editing with CRISPR/Cas9.Aquaculture,2020; 529. https://doi.org/10.1016/j.aquaculture.2020.735672.

[85] PanH, YuH, RaviV, LiC, LeeAP, LianMM, TayB-H, BrennerS, WangJ, YangH, ZhangG, VenkateshB. The genome of the largest bony fish, ocean sunfish (Mola mola), provides insights into its fast growth rate. Gigascience., 2016, 5(1): 36

[86] PengJ, LiQ, XuL, WeiP, HeP, ZhangX, ZhangL, GuanJ, ZhangX, LinY, GuiJ, ChenX. Chromosome-level analysis of the Crassostrea hongkongensis genome reveals extensive duplication of immune-related genes in bivalves. Mol Ecol Resour., 2020, 20(4): 980-994

[87] RanZ, LiZ, YanX, LiaoK, KongF, ZhangL, CaoJ, ZhouC, ZhuP, HeS, HuangW, XuJ. Chromosome-level genome assembly of the razor clam Sinonovacula constricta (Lamarck, 1818). Mol Ecol Resour, 2019, 19(6): 1647-1658

[88] RenX, LvJ, LiuM, WangQ, ShaoH, LiuP, LiJ. A chromosome-level genome of the kuruma shrimp (Marsupenaeus japonicus) provides insights into its evolution and cold-resistance mechanism. Genomics., 2022, 114(3): 110373

[89] SeranoJM, MartinA, LiubicichDM, JarvisE, BruceHS, LaK, BrowneWE, GrimwoodJ, PatelNH. Comprehensive analysis of Hox gene expression in the amphipod crustacean Parhyale hawaiensis. Dev Biol., 2016, 409(1): 297-309

[90] ShanX, XuT, MaZ, ZhangX, RuanZ, ChenJ, ShiQ, YouX. Genome-wide association improves genomic selection for ammonia tolerance in the orange-spotted grouper (Epinephelus coioides). Aquaculture, 2021, 533

[91] ShanX, ZhangX, RuanZ, ChenJ, ShiQ, XuJ, YouX. Genomic selection of orange-spotted grouper (Epinephelus coioides) based on multiplex PCR enrichment capture sequencing. Aquacult Fish., 2023, 8(6): 681-688

[92] ShaoCW, BaoBL, XieZY, ChenXY, LiB, JiaXD, YaoQ, OrtiG, LiWH, LiXH, HamreK, XuJ, WangL, ChenFY, TianYS, SchreiberAM, WangN, WeiF, ZhangJ, DongZD, GaoL, GaiJW, SakamotoT, MoSD, ChenWJ, ShiQ, LiH, XiuY, LiY, XuW, ShiZ, ZhangG, PowerDM, WangQ, SchartlM, ChenS. The genome and transcriptome of Japanese flounder provide insights into flatfish asymmetry. Nat Genet., 2017, 49(1): 119-124

[93] ShaoC, LiC, WangN, QinY, XuW, LiuQ, ZhouQ, ZhaoY, LiX, LiuS, ChenX, MahboobS, LiuX, ChenS. Chromosome-level genome assembly of the spotted sea bass, Lateolabrax maculatus. Gigascience, 2018, 7(11): giy114

[94] ShenR, FanJ-B, CampbellD, ChangW, ChenJ, DoucetD, YeakleyJ, BibikovaM, Wickham GarciaE, McBrideC, SteemersF, GarciaF, KermaniBG, GundersonK, OliphantA. High-throughput SNP genotyping on universal bead arrays. Mut Res Fund Mol Mechan Mutagen., 2005, 573(1–2): 70-82

[95] SongH, DongT, HuM, YanX, XuS, HuH. First single-step genomic prediction and genome-wide association for body weight in Russian sturgeon (Acipenser gueldenstaedtii). Aquaculture, 2022, 561

[96] Song H, Guo X, Sun L, Wang Q, Han F, Wang H, Wray G A, Davidson P, Wang Q, Hu Z, Zhou C, Yu Z, Yang M, Feng J, Shi P, Zhou Y, Zhang L, Zhang T. The hard clam genome reveals massive expansion and diversification of inhibitors of apoptosis in Bivalvia. Bmc Biol. 2021;19(1). https://doi.org/10.1186/s12915-020-00943-9.

[97] Song W T, Li Y Z, Zhao Y W, Liu Y, Niu Y Z, Pang R Y, Miao G D, Liao X L, Shao C W, Gao F T, Chen S L. Construction of a High-Density Microsatellite Genetic Linkage Map and Mapping of Sexual and Growth-Related Traits in Half-Smooth Tongue Sole (Cynoglossus semilaevis). Plos One. 2012;7(12). https://doi.org/10.1371/journal.pone.0052097.

[98] SuH, LiH, WangS, WangY, BaoZ. Performance comparison of two efficient genomic selection methods (gsbay & MixP) applied in aquacultural organisms. J Ocean Univ China., 2017, 16(1): 137-144

[99] Sun L, Jiang C, Su F, Cui W, Yang H. Chromosome-level genome assembly of the sea cucumber Apostichopus japonicus. Scientific Data. 2023;10(1). https://doi.org/10.1038/s41597-023-02368-9.

[100] SunS, LiW, XiaoS, LinA, HanZ, CaiM, WangZ. Genetic sex identification and the potential sex determination system in the yellow drum (Nibea albiflora). Aquaculture, 2018, 492: 253-258

[101] TangB, ZhangD, LiH, JiangS, ZhangH, XuanF, GeB, WangZ, LiuY, ShaZ, ChengY, JiangW, JiangH, WangZ, WangK, LiC, SunY, SheS, QiuQ, WangW, LiX, LiY, LiuQ, RenY. Chromosome-level genome assembly reveals the unique genome evolution of the swimming crab (Portunus trituberculatus). Gigascience., 2020, 9(1): giz161

[102] TengW, FuH, LiZ, ZhangQ, XuC, YuH, KongL, LiuS, LiQ. Parallel evolution in Crassostrea oysters along the latitudinal gradient is associated with variation in multiple genes involved in adipogenesis. Mole Ecol., 2023, 32(19): 5276-5287

[103] VallejoRL, LeedsTD, FragomeniBO, GaoG, HernandezAG, MisztalI, WelchTJ, WiensGD, PaltiY. Evaluation of Genome-Enabled Selection for Bacterial Cold Water Disease Resistance Using Progeny Performance Data in Rainbow Trout: Insights on Genotyping Methods and Genomic Prediction Models. Front Genet, 2016, 7: 96

[104] WangD, ChenX, ZhangX, LiJ, YiY, BianC, ShiQ, LinH, LiS, ZhangY, YouX. Whole genome sequencing of the giant grouper (Epinephelus lanceolatus) and high-throughput screening of putative antimicrobial peptide genes. Mar Drugs., 2019, 17(9): 56-65

[105] WangN, LiuY, WangR, ChenS. The linkage of cell cycle and DNA replication with growth difference in female Chinese tongue sole (Cynoglossus semilaevis): Analysis from transcriptomic study and WGCNA. Comp Biochem Physiol Part D Genomics Proteomics., 2021, 39: 100833

[106] WangJ, MiaoL, ChenB, ZhaoJ, KeQ, PuF, ZhouT, XuP. Development and evaluation of liquid SNP array for large yellow croaker (Larimichthys crocea). Aquaculture, 2023, 563

[107] WangJ, ZhaoJ, TongB, KeQ, BaiY, GongJ, ZengJ, DengY, LanB, ZhouT, XuP. Effects of artificial mating on genomic selection of resistance against Cryptocaryon irritans in large yellow croaker. Aquaculture., 2022, 561: 738617

[108] WangL, LiZ, LiuY, ChenS, LiL, DuanP, WangX, LiW, WangQ, ZhaiJ, TianY. A chromosome-level genome assembly of the potato grouper (Epinephelus tukula). Genomics, 2022, 114(5): 110473

[109] WangL, TanXG, WuZH, WangLJ, JiaoS, ZouYX, JiGL, YouF. Targeted mutagenesis in the olive flounder (Paralichthys olivaceus) using the CRISPR/Cas9 system with electroporation. Biologia., 2021, 76(4): 1297-1304

[110] WangQ, RenX, LiuP, LiJ, LvJ, WangJ, ZhangH, WeiW, ZhouY, HeY, LiJ. Improved genome assembly of Chinese shrimp (Fenneropenaeus chinensis) suggests adaptation to the environment during evolution and domestication. Mol Ecol Resour., 2022, 22(1): 334-344

[111] WangQ, YuY, LiF, ZhangX, XiangJ. Predictive ability of genomic selection models for breeding value estimation on growth traits of Pacific white shrimp Litopenaeus vannamei. Chin J Oceanol Limn., 2016, 35(5): 1221-1229

[112] WangQ, YuY, ZhangQ, ZhangX, HuangH, XiangJ, LiF. Evaluation on the genomic selection in Litopenaeus vannamei for the resistance against Vibrio parahaemolyticus. Aquaculture., 2019, 505: 212-216

[113] WangS, ZhangJ, JiaoW, LiJ, XunX, SunY, GuoX, HuanP, DongB, ZhangL, HuX, SunX, WangJ, ZhaoC, WangY, WangD, HuangX, WangR, LvJ, LiY, ZhangZ, LiuB, LuW, HuiY, LiangJ, ZhouZ, HouR, LiX, LiuY, LiH, NingX, LinY, ZhaoL, XingQ, DouJ, LiY, MaoJ, GuoH, DouH, LiT, MuC, JiangW, FuQ, FuX, MiaoY, LiuJ, YuQ, LiR, LiaoH, LiX, KongY, JiangZ, ChourroutD, LiR, BaoZ. Scallop genome provides insights into evolution of bilaterian karyotype and development. Nat Ecol Evol., 2017, 1(5): 120

[114] WangY, SunG, ZengQ, ChenZ, HuX, LiH, WangS, BaoZ. Predicting Growth Traits with Genomic Selection Methods in Zhikong Scallop (Chlamys farreri). Mar Biotechnol, 2018, 20(6): 769-779

[115] Wang Y, Wen X, Zhang X, Fu S, Liu J, Tan W, Luo M, Liu L, Huang H, You X, Luo J, Chen F. Chromosome Genome Assembly of the Leopard Coral Grouper (Plectropomus leopardus) With Nanopore and Hi-C Sequencing Data.Frontiers in Genetics,2020; 11. https://doi.org/10.3389/fgene.2020.00876.

[116] WuB, ChenX, YuM, RenJ, HuJ, ShaoC, ZhouL, SunX, YuT, ZhengY, WangY, WangZ, ZhangH, FanG, LiuZ. Chromosome-level genome and population genomic analysis provide insights into the evolution and environmental adaptation of Jinjiang oyster Crassostrea ariakensis. Mol Ecol Resour., 2021, 22(4): 1529-1544

[117] WuC, ZhangD, KanM, LvZ, ZhuA, SuY, ZhouD, ZhangJ, ZhangZ, XuM, JiangL, GuoB, WangT, ChiC, MaoY, ZhouJ, YuX, WangH, WengX, JinJG, YeJ, HeL, LiuY. The draft genome of the large yellow croaker reveals well-developed innate immunity. Nat Commun., 2014, 5: 5227

[118] Wu F, Cheng C, Li X, Ren C, Luo P, Jiang X, E Z, Zhang X, Hu C, Chen T. Identification of sex-specific molecular markers and development of PCR-based sex detection techniques in tropical sea cucumber (Stichopus monotuberculatus). Aquaculture. 2022;547:737458. https://doi.org/10.1016/j.aquaculture.2021.737458.

[119] XiaoY, XiaoZ, MaD, LiuJ, LiJ. Genome sequence of the barred knifejaw Oplegnathus fasciatus (Temminck & Schlegel, 1844): the first chromosome-level draft genome in the family Oplegnathidae. Gigascience., 2019, 8(3): giz013

[120] XieQP, ZhanW, ShiJZ, LiuF, NiuBL, HeX, LiuM, WangJ, LiangQQ, XieY, XuP, WangX, LouB. Whole-genome assembly and annotation for the little yellow croaker (Larimichthys polyactis) provide insights into the evolution of hermaphroditism and gonochorism. Mol Ecol Resour., 2023, 23(3): 632-658

[121] XieX, TengW, YuZ, LiD, YangM, ZhangH, ZhengJ, LiH, SunY, LiuX, ZhouZ, ZhangX, DuS, LiQ, ChangY, ZhangM, WangQ. Chromosome-level genome assembly of sea scallop Placopecten magellanicus provides insights into the genetic characteristics and adaptive evolution of large scallops. Genomics., 2023, 115(6): 110747

[122] XuDD, LouB, XuHX, LiSL, GengZ. Isolation and Characterization of Male-Specific DNA Markers in the Rock Bream Oplegnathus fasciatus. Mar Biotechnol., 2013, 15(2): 221-229

[123] Xu R, Martelossi J, Smits M, Iannello M, Peruzza L, Babbucci M, Milan M, Dunham J P, Breton S, Milani L, Nuzhdin S V, Bargelloni L, Passamonti M, Ghiselli F. Multi-tissue RNA-Seq Analysis and Long-read-based Genome Assembly Reveal Complex Sex-specific Gene Regulation and Molecular Evolution in the Manila Clam. Genome Biol Evol. 2022a;14(12). https://doi.org/10.1093/gbe/evac171.

[124] XuS, XiaoS, ZhuS, ZengX, LuoJ, LiuJ, GaoT, ChenN. A draft genome assembly of the Chinese sillago (Sillago sinica), the first reference genome for Sillaginidae fishes. Gigascience., 2018, 7(9): giy108

[125] Xu T, Xu G, Che R, Wang R, Wang Y, Li J, Wang S, Shu C, Sun Y, Liu T, Liu J, Wang A, Han J, Chu Q, Yang Q. The genome of the miiuy croaker reveals well-developed innate immune and sensory systems. Sci Rep. 2016;6(1). https://doi.org/10.1038/srep21902.

[126] Xu X, Chen Z, Liu C, Xu W, Xu H, Chen S. Chromosome-level genome assembly of the Verasper variegatus provides insights into left eye migration.Front Mar Sci,2022b;9. https://doi.org/10.3389/fmars.2022.1045052.

[127] Xu X, Shao C, Xu H, Zhou Q, You F, Wang N, Li W, Li M, Chen S. Draft genomes of female and male turbot Scophthalmus maximus.Sci Data,2020; 7(1). https://doi.org/10.1038/s41597-020-0426-6.

[128] YanMZ, LiBJ, WangJY, BaiYL, KeQZ, ZhouT, XuP. Disruption of mstn Gene by CRISPR/Cas9 in Large Yellow Croaker (Larimichthys crocea). Mar Biotechnol, 2022, 24(4): 681-689

[129] YanX, NieH, HuoZ, DingJ, LiZ, YanL, JiangL, MuZ, WangH, MengX, ChenP, ZhouM, RbbaniMG, LiuG, LiD. Clam Genome Sequence Clarifies the Molecular Basis of Its Benthic Adaptation and Extraordinary Shell Color Diversity. Iscience, 2019, 19: 1225-1237

[130] YangB, ZhiC, LiP, XuC, LiQ, LiuS. Genomic selection accelerates genetic improvement of resistance to Vibriosis in the Pacific, Crassostrea gigas. oyster. Aquaculture., 2024, 584: 740679

[131] YangJL, FengDD, LiuJ, XuJK, ChenK, LiYF, ZhuYT, LiangX, LuY. Chromosome-level genome assembly of the hard-shelled mussel Mytilus coruscus, a widely distributed species from the temperate areas of East Asia. Gigascience., 2021, 10(4): giab024

[132] Yang Y, Wu L N, Chen J F, Wu X, Xia J H, Meng Z N, Liu X C, Lin H R. Whole-genome sequencing of leopard coral grouper ( Plectropomus leopardus) and exploration of regulation mechanism of skin color and adaptive evolution. Zool Res. 2020;41(3):328–340. https://doi.org/10.24272/j.issn.2095-8137.2020.038.

[133] Yang Y, Zeng L, Wang T, Wu L, Wu X, Xia J, Meng Z, Liu X. Assembly of Genome and Resequencing Provide Insights into Genetic Differentiation between Parents of Hulong Hybrid Grouper (Epinephelus fuscoguttatus female symbol x E. lanceolatus male symbol). Int J Mol Sci. 2023;24(15). https://doi.org/10.3390/ijms241512007.

[134] You X, Bian C, Zan Q, Xu X, Liu X, Chen J, Wang J, Qiu Y, Li W, Zhang X, Sun Y, Chen S, Hong W, Li Y, Cheng S, Fan G, Shi C, Liang J, Tom Tang Y, Yang C, Ruan Z, Bai J, Peng C, Mu Q, Lu J, Fan M, Yang S, Huang Z, Jiang X, Fang X, Zhang G, Zhang Y, Polgar G, Yu H, Li J, Liu Z, Zhang G, Ravi V, Coon S L, Wang J, Yang H, Venkatesh B, Wang J, Shi Q. Mudskipper genomes provide insights into the terrestrial adaptation of amphibious fishes. Nat Commun. 2014;5(1). https://doi.org/10.1038/ncomms6594.

[135] YuH, LiHJ, LiQ, XuR, YueCY, DuSJ. Targeted Gene Disruption in Pacific Oyster Based on CRISPR/Cas9 Ribonucleoprotein Complexes. Mar Biotechnol, 2019, 21(3): 301-309

[136] YuM, XieQ-P, WeiF-L, WuX-F, XuW-T, ZhanW, LiuF, GuoD-D, NiuB-L, LouB. Development and identification of a sex-specific molecular marker in Dai-qu stock large yellow croaker (Larimichthys crocea). Aquaculture, 2022, 555: 738172

[137] YuY, LuoZ, WangQ, ZhangQ, ZhangX, XiangJ, LiF. Development of high throughput SNP genotyping approach using target sequencing in Pacific white shrimp and its application for genetic study. Aquaculture., 2020, 528: 735549

[138] ZengJ, ZhaoJ, WangJ, BaiY, LongF, DengY, JiangP, XiaoJ, QuA, TongB, WangM, LiuW, PuF, LiY, XuP. Genetic linkage between swimming performance and disease resistance enables multitrait breeding strategies in large yellow croaker. Agricult Commun., 2023, 1(2): 100019

[139] ZhangC, WenH, ZhangY, ZhangK, QiX, LiY. First genome-wide association study and genomic prediction for growth traits in spotted sea bass (Lateolabrax maculatus) using whole-genome resequencing. Aquaculture., 2023, 566: 739194

[140] Zhang D-C, Guo L, Guo H-Y, Zhu K-C, Li S-Q, Zhang Y, Zhang N, Liu B-S, Jiang S-G, Li J-T. Chromosome-level genome assembly of golden pompano (Trachinotus ovatus) in the family Carangidae.Sci Data,2019; 6(1). https://doi.org/10.1038/s41597-019-0238-8.

[141] ZhangG, FangX, GuoX, LiL, LuoR, XuF, YangP, ZhangL, WangX, QiH, XiongZ, QueH, XieY, HollandPW, PapsJ, ZhuY, WuF, ChenY, WangJ, PengC, MengJ, YangL, LiuJ, WenB, ZhangN, HuangZ, ZhuQ, FengY, MountA, HedgecockD, XuZ, LiuY, Domazet-LosoT, DuY, SunX, ZhangS, LiuB, ChengP, JiangX, LiJ, FanD, WangW, FuW, WangT, WangB, ZhangJ, PengZ, LiY, LiN, WangJ, ChenM, HeY, TanF, SongX, ZhengQ, HuangR, YangH, DuX, ChenL, YangM, GaffneyPM, WangS, LuoL, SheZ, MingY, HuangW, ZhangS, HuangB, ZhangY, QuT, NiP, MiaoG, WangJ, WangQ, SteinbergCE, WangH, LiN, QianL, ZhangG, LiY, YangH, LiuX, WangJ, YinY, WangJ. The oyster genome reveals stress adaptation and complexity of shell formation. Nature., 2012, 490(7418): 49-54

[142] ZhangX, SunL, YuanJ, SunY, GaoY, ZhangL, LiS, DaiH, HamelJ-F, LiuC, YuY, LiuS, LinW, GuoK, JinS, XuP, StoreyKB, HuanP, ZhangT, ZhouY, ZhangJ, LinC, LiX, XingL, HuoD, SunM, WangL, MercierA, LiF, YangH, XiangJ. The sea cucumber genome provides insights into morphological evolution and visceral regeneration. Plos Biol., 2017, 15(10): e2003790

[143] ZhangX, YuanJ, SunY, LiS, GaoY, YuY, LiuC, WangQ, LvX, ZhangX, MaKY, WangX, LinW, WangL, ZhuX, ZhangC, ZhangJ, JinS, YuK, KongJ, XuP, ChenJ, ZhangH, SorgeloosP, SagiA, Alcivar-WarrenA, LiuZ, WangL, RuanJ, ChuKH, LiuB, LiF, XiangJ. Penaeid shrimp genome provides insights into benthic adaptation and frequent molting. Nat Commun., 2019, 10(1): 356

[144] ZhangZ, ZhangK, ChenS, ZhangZ, ZhangJ, YouX, BianC, XuJ, JiaC, QiangJ, ZhuF, LiH, LiuH, ShenD, RenZ, ChenJ, LiJ, GaoT, GuR, XuJ, ShiQ, XuP. Draft genome of the protandrous Chinese black porgy Acanthopagrus schlegelii. Gigascience., 2018, 7(4): 1-7

[145] Zhao J, Bai H, Ke Q, Li B, Zhou Z, Wang H, Chen B, Pu F, Zhou T, Xu P. Genomic selection for parasitic ciliate Cryptocaryon irritans resistance in large yellow croaker. Aquaculture. 2021a;531. https://doi.org/10.1016/j.aquaculture.2020.735786.

[146] ZhaoM, WangW, ZhangF, MaC, LiuZ, YangMH, ChenW, LiQ, CuiM, JiangK, FengC, LiJT, MaL. A chromosome-level genome of the mud crab (Scylla paramamosain estampador) provides insights into the evolution of chemical and light perception in this crustacean. Mol Ecol Resour., 2021, 21(4): 1299-1317

[147] ZhengW, ChenY, ZhangT, LiW, QuJ, ChenS, XuX. Identification of two female-specific DNA markers in Verasper variegatus. Aquaculture., 2024, 588: 740910

[148] Zhong S, Liu X, Ma X, Chen X, Jiang Y, Zeng M, Zhao L, Huang L, Huang G, Zhao Y, Liu H, Qiao Y. Chromosomal-level genome assembly and annotation of the tropical sea cucumber Holothuria scabra. Sci Data. 2024;11(1). https://doi.org/10.1038/s41597-024-03340-x.

[149] ZhouM, YuanY, ZhangY, ZhangW, ZhouR, JiJ, WuH, ZhaoY, ZhangD, LiuB, JiangD, WangZ, FangM. The study of the genomic selection of white gill disease resistance in large yellow croaker (Larimichthys crocea). Aquaculture, 2023, 574: 739682

[150] Zhou Q, Chen Y-d, Lu S, Liu Y, Xu W-t, Li Y-z, Wang L, Wang N, Yang Y-m, Chen S-l. Development of a 50K SNP Array for Japanese Flounder and Its Application in Genomic Selection for Disease Resistance. Engineering-Prc. 2021a;7(3):406–411. https://doi.org/10.1016/j.eng.2020.06.017.

[151] ZhouQ, GaoH, XuH, LinH, ChenS. A Chromosomal-scale Reference Genome of the Kelp Grouper Epinephelus moara. Mar Biotechnol (NY), 2021, 23(1): 12-16

[152] ZhouQ, GaoH, ZhangY, FanG, XuH, ZhaiJ, XuW, ChenZ, ZhangH, LiuS, NiuY, LiW, LiW, LinH, ChenS. A chromosome-level genome assembly of the giant grouper (Epinephelus lanceolatus) provides insights into its innate immunity and rapid growth. Mol Ecol Resour., 2019, 19(5): 1322-1332

[153] ZhouQ, GuoX, HuangY, GaoH, XuH, LiuS, ZhengW, ZhangT, TianC, ZhuC, LinH, ChenS. De novo sequencing and chromosomal-scale genome assembly of leopard coral grouper, Plectropomus leopardus.. Mol Ecol Resour., 2020, 20(5): 1403-1413

[154] ZhouQ, LuS, LiuY, ZhouB, ChenS. Development of a 20 K SNP array for the leopard coral grouper. Plectropomus leopardus. Aquaculture., 2024, 578: 740079

[155] ZhouQ, WangJ, LiJ, ChenZ, WangN, LiM, WangL, SiY, LuS, CuiZ, LiuX, ChenS. Decoding the fish genome opens a new era in important trait research and molecular breeding in China. Sci China Life Sci, 2024

[156] Zhou T, Chen B H, Ke Q Z, Zhao J, Wang J Y, Bai Y L, Pu F, Pan Y, Chen J, Zheng W Q, Xu P. Development and evaluation of a breeding array for genomic selection of large yellow croaker (Larmichthys crocea). J Fish Sci China. 2022;29(01):41–48.  https://doi.org/10.12264/jfsc2021-0243. (in Chinese).

[157] Zhou T, Chen B, Ke Q, Zhao J, Pu F, Wu Y, Chen L, Zhou Z, Bai Y, Pan Y, Gong J, Zheng W, Xu P. Development and Evaluation of a High-Throughput Single-Nucleotide Polymorphism Array for Large Yellow Croaker (Larimichthys crocea). Front Genet. 2020b;11. https://doi.org/10.3389/fgene.2020.571751.

[158] Zhou Z, Liu B, Chen B, Shi Y, Pu F, Bai H, Li L, Xu P. The sequence and de novo assembly of Takifugu bimaculatus genome using PacBio and Hi-C technologies. Sci Data. 2019b;6(1). https://doi.org/10.1038/s41597-019-0195-2.

[159] ZhuX, NiP, XingQ, WangY, HuangX, HuX, HuJ, WuX-L, BaoZ. Genomic prediction of growth traits in scallops using convolutional neural networks. Aquaculture., 2021, 545: 737171

[160] Zou M, Xia Z. Hyper-seq: A novel, effective, and flexible marker-assisted selection and genotyping approach. Innovation. 2022;3(4). https://doi.org/10.1016/j.xinn.2022.100254.