Effect of Rice Bran Husk Substrate on the Production of Asparginase by Streptomyces sp. Derived from Termite Mound

Abstract

An important criterion for the potency assessment is that the source of cheap substrate for L-asparaginase production is much needed. Isolation of novel actinobacteria from a termite mound was performed by conventional pretreatment isolation and enzyme screening by plate assay method to compare the effect of carbon and substrate over L-asparaginase produced by submerged fermentation state with minimal medium. The enzyme production was studied by the Nesslerization method. Following heat treatment, the results indicated that the isolate decreased the eubacterial colony-forming unit. Out of the ten pre-heated samples, one soil tested positive for actinobacteria and nine tested negative. The isolate detected as asparaginase-positive was annotated as TS5 morphologically showed aerial and substrate mycelia formation and was identified as Streptomyces sp. The production of L-asparaginase among four different carbons revealed that lactose was an inducer. Further the cheap substrate such as rice bran is evaluated under substrate state fermentation and a good result on enzyme production is noted on both defatted and raw rice bran. The highest peak activity of the enzyme production (183 ± 9.11 U/mL) was attained at 10% raw rice bran with 66.3 ± 0.26 mg/mL protein. These results provide evidence that agro-waste substrate within the investigated parameters increased the LA generation. These findings are expected to promote the industrial utilization of important actinomycetes from termite mound.

Keywords

agro-waste / substrate fermentation / rice bran / asparaginase / cancer

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