Genetic predictors regarding of infection-dependent pregnancy loss

Abstract

A retrospective comparative cohort study investigated the prevalence of genetic variants are associated with immune hyper-response of the immune system in women (n = 96) with a history of infected miscarriage. The comparison groups subjects was 76 women of the same age with 2 or more normal pregnancies in history.

The frequency of gene polymorphism in patients in all patients of the main group and the comparison group: the G894T polymorphism (Glu298Asp, rs1799983) of the Nos3 gene, the C1234T (p.Leu412Phe, rs3775291) polymorphism of the Tlr3 gene, the C3954T (rs1143644) and C-511T (rs16944) polymorphisms of the IL1B gene, and G-308A (rs1800629) and G238A (rs361525) of the Tnf gene. For comparative statistical analysis, we used the chi-square test and Fisher’s exact test with the determination of relative risk and confidence interval (CI).

Comparative analysis showed that polymorphism in defined gene was associated with an increased the relative risk of infected miscarriage: T/T genotype of the Tlr3 gene (C1234T), the C allele of the Il1b gene (C3954T), and the A allele of the Tnf gene (G238A) increases the relative risk of infected miscarriage by 3.345 times (95% confidence interval 1.594–7.017; chi-square test = 10.779, p = 0.002), 5.077 times (95% confidence interval 2.743–9.396; chi-square test = 30.272, p < 0.001) and 2.958 times (95% confidence interval 1.451–6.032; chi-square test = 9.533, p = 0.003), respectively.

Keywords

cytokines / genetic polymorphisms / haplotype / infected miscarriage / polymerase chain reaction / pregnancy loss / toll-like receptor 3

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