State of intestinal microbiocenosis and quality of life in patients with thyroid diseases

Abstract

BACKGROUND: Non-invasive methods for diagnosing thyroid pathology with an inapparent, asymptomatic or clinically non-specific course is an urgent task of thyroidology today. It seems promising for such diagnostics to use a combination of mass spectrometry of microbial markers of the human intestinal microbiota with an assessment of the quality of life using the 36-Item Short Form Survey (SF-36) questionnaire.

AIM: to study the features of intestinal microbiocenosis and subjective assessment of the quality of life in patients with thyroid diseases.

MATERIALS AND METHODS: 21 patients were examined (8 men and 13 women, median age 40,5 [31,75; 54] years, respectively; 15 with thyroid disease of varying degrees of clinical manifestations, 6 without thyroid disease). In addition to the standard clinical and laboratory-instrumental examination, the patients underwent a study of the contents of the large intestine by the method of chromato-mass spectrometry of microbial markers. Through the use of the SF-36 methodology, an assessment of the quality of life was implemented. Descriptive, nonparametric statistics were used in the work, differences in relative frequency values were determined using Fisher’s exact test. The presentation of the results was carried out using range diagrams, compactly depicting a one-dimensional probability distribution, tables.

RESULTS: Differences in the composition of the microbiota of the control group and the study group for Alcaligenes spp., Staphylococcus spp. were revealed, a downward trend in the level of Megamonas hypermegale, Peptostreptococcus anaerobius in the main group is determined. A tendency revealing a decrease in the quality of life in the study group compared with the control in terms of social functioning, role emotional functioning was revealed.

CONCLUSIONS: Pathology of the thyroid gland is accompanied by deviations in the composition of the fecal microbiota, determined by the method of mass spectrometry of microbial markers. Characteristic features of changes in the intestinal microbiome are a decrease in the level of Alcaligenes spp., Staphylococcus spp., a tendency to decrease in the titers of Megamonas hypermegale, Peptostreptococcus anaerobius. Violation of the endocrine function of the thyroid gland can cause a deterioration in the quality of life, which is manifested by a tendency to reduce social functioning, role-playing emotional functioning.

Keywords

gut microbiome / gut microbiota / mass spectrometry / microbial markers / quality of life / questionnaire / thyroid disease

References

[1] Kovaleva NI, Korneeva NA. Autoimmune thyroiditis. Modern methods of diagnostics and treatment. Medicinal herald. 2006;3(7(23)):22–26. (In Russ.)

[2] Ковалева Н.И., Корнеева Н.А. Аутоиммунный тиреоидит. Современные методы диагностики и лечения // Лекарственный вестник. 2006. Т. 3, № 7 (23). С. 22–26.

[3] Healthcare in Russia. 2021: Stat. sb. Moscow: Rosstat; 2021. 171 p. (In Russ.) Available at: https://rosstat.gov.ru/storage/mediabank/Zdravoohran-2021.pdf (accessed 01.04.2022).

[4] Здравоохранение в России. 2021: Стат. сб. М.: Росстат, 2021. 171 с. Доступен по: https://rosstat.gov.ru/storage/mediabank/Zdravoohran-2021.pdf (дата обращения 01.04.2022).

[5] Troshina EA, Platonova NM, Panfilova EA. Dynamics of epidemiological indicators of thyroid pathology in the population of the Russian Federation: analytical report for the period 2009–2018. Problems of Endocrinology. 2021;67(2):10–19. (In Russ.) DOI: 10.14341/probl12433

[6] Трошина Е.А., Платонова Н.М., Панфилова Е.А. Аналитический обзор результатов мониторинга основных эпидемиологических характеристик йододефицитных заболеваний у населения Российской Федерации за период 2009–2018 гг. // Проблемы эндокринологии. 2021. Т. 67, № 2. С. 10–19. 10.14341/probl12433

[7] Kasatkina EP. Autoimmune thyroiditis: diagnosis and treatment (Discussion of the paper by V.V. Fadeev, G.A. Melnichenko, G.A. Gerasimov “Autoimmune Thyroiditis: First Step to Consensus”). Problems of Endocrinology. 2002;48(3):3–6. (In Russ.) DOI: 10.14341/probl11596

[8] Касаткина Э.П. Аутоиммунный тиреоидит: диагностика и лечение (дискуссия по поводу статьи В.В. Фадеева, Г.А. Мельниченко, Г.А. Герасимова «Аутоиммунный тиреоидит: первый шаг к консенсусу») // Проблемы эндокринологии. 2002. Т. 48, № 3. С. 3–6. DOI: 10.14341/probl11596

[9] Kucher IA, Chapliev IY. Morphofunctional changes of the thyroid gland in its dysfunction and pathology. Russian Military Medical Academy Reports. 2020;39(S1–2):99–101. (In Russ.) DOI: 10.17816/rmmar43373

[10] Кучер И.А, Чаплиев И.Ю. Морфофункциональные изменения щитовидной железы при ее дисфункции и патологии // Известия Российской Военно-медицинской академии. 2020. Т. 39, № S1–2. С. 99–101. DOI: 10.17816/rmmar43373

[11] Platonova NM, Makolina NP, Rybakova AA, Troshina EA. Autoimmune thyroiditis and pregnancy: changes in the modern diagnostic and therapeutic paradigms. Russian Journal of Human Reproduction. 2020;26(1):29–38. (In Russ.) DOI: 10.17116/repro20202601129

[12] Платонова Н.М., Маколина Н.П., Рыбакова А.А., Трошина Е.А. Аутоиммунный тиреоидит и беременность: изменения в современных лечебно-диагностических парадигмах // Проблемы репродукции. 2020. Т. 26, № 1. С. 29–38. DOI: 10.17116/repro20202601129

[13] Khvostovoy VV, Kiselev IL, Sychov MD, et al. The course, diagnosis, and treatment of thyroid tumors in the presence of chronic autoimmune thyroiditis. Diagnosis and treatment of head tumors and tumors. 2011;(4):5–11. (In Russ.)

[14] Хвостовой В.В., Киселев И.Л., Cычов М.Д., и др. Особенности течения, диагностики и лечения опухолей щитовидной железы на фоне хронического аутоиммунного тиреоидита // Диагностика и лечение опухолей головы и шеи. 2011. № 4. С. 5–11.

[15] Dreval’ AV, Nechaeva OA, Garbuzov PI, et al. Quality of life indices and psychoemotional status of the patients presenting with diffuse toxic goiter during the long-term follow-up after iodine radiotherapy. Problems of Endocrinology. 2013;59(3):13–18. (In Russ.)

[16] Древаль А.В., Нечаева О.А., Гарбузов П.И., и др. Показатели качества жизни, психоэмоциональный статус больных диффузным токсическим зобом в отдаленные сроки после радиойодтерапии // Проблемы эндокринологии. 2013. Т. 59, № 3. С. 13–18.

[17] Docimo G, Cangiano A, Romano R, et al. The Human Microbiota in Endocrinology: Implications for Pathophysiology, Treatment, and Prognosis in Thyroid Diseases. Frontiers in Endocrinology. 2020;11: 1–7. DOI: 10.3389/fendo.2020.586529

[18] Docimo G., Cangiano A., Romano R., et al. The Human Microbiota in Endocrinology: Implications for Pathophysiology, Treatment, and Prognosis in Thyroid Diseases // Frontiers in Endocrinology. 2020. Vol. 11. P. 1–7. DOI: 10.3389/fendo.2020.586529

[19] Knezevic J, Starchl C, Berisha A, Amrein K. Thyroid-Gut-Axis: How Does the Microbiota Influence Thyroid Function? Nutrients. 2020;12(6):1769. DOI: 10.3390/nu12061769

[20] Knezevic J., Starchl C., Berisha A., Amrein K. Thyroid-Gut-Axis: How Does the Microbiota Influence Thyroid Function? // Nutrients. 2020. Vol. 12, No. 6. P. 1769. DOI: 10.3390/nu12061769

[21] Bargiel P, Szсzuko M, Stachowska L, et al. Microbiome Metabolites and Thyroid Dysfunction. Journal of Clinical Medicine. 2021;10(16):3609. DOI: 10.3390/jcm10163609

[22] Bargiel P., Szсzuko M., Stachowska L., et al. Microbiome Metabolites and Thyroid Dysfunction // Journal of Clinical Medicine. 2021.Vol. 10, No. 16. P. 3609. DOI: 10.3390/jcm10163609

[23] Zhou H, Yuan Y, Wang H, et al. Gut Microbiota: A Potential Target for Cancer Interventions. Cancer Management and Research. 2021;13:8281–8296. DOI: 10.2147/CMAR.S328249

[24] Zhou H., Yuan Y., Wang H., et al. Gut Microbiota: A Potential Target for Cancer Interventions // Cancer Management and Research. 2021. Vol. 13. P. 8281–8296. DOI: 10.2147/CMAR.S328249

[25] Zhu Q, Hou Q, Huang S, et al. Compositional and genetic alterations in Graves’ disease gut microbiome reveal specific diagnostic biomarkers. ISME J. 2021;15(11):3399–3411. DOI: 10.1038/s41396-021-01016-7

[26] Zhu Q., Hou Q., Huang S., et al. Compositional and genetic alterations in Graves’ disease gut microbiome reveal specific diagnostic biomarkers // ISME J. 2021. Vol. 15, No. 11. P. 3399–3411. DOI: 10.1038/s41396-021-01016-7

[27] Voroshilina ES, Zornikov DL, Panacheva EA. Evaluation of the ejaculate microbiota by real-time PCR and culture-based technique. Bulletin of the RSMU. 2019;1:44–49. (In Russ.) DOI: 10.24075/vrgmu.2019.009

[28] Ворошилина Е.С., Зорников Д.Л., Паначева Е.А. Сравнительное исследование микробиоты эякулята методом количественной ПЦР и культуральным методом // Вестник РГМУ. 2019. № 1. С. 44–49. DOI: 10.24075/vrgmu.2019.009

[29] Alekseeva AE, Brusningina NF. Metagenomic research and diagnostics of infectious diseases. Journal of Microbiology, Epidemiology and Immunobiology. 2015;(2):81–89. (In Russ.)

[30] Алексеева А.Е., Бруснигина Н.Ф. Метагеномные исследования и диагностика инфекционных заболеваний // Журнал микробиологии, эпидемиологии и иммунобиологии. 2015. № 2. С. 81–89.

[31] Malyshev VV, Razumova DN, Shayakhmetov LK. Metagenomic analysis of microbial contaminants in the hospital environment and the 16S gene. Medicine: theory and practice. 2019;4(S):333–334. (In Russ.)

[32] Малышев В.В., Разумова Д.Н., Шаяхметов Л.К. Метагеномный анализ микробных контаминантов больничной среды и ген 16S PРНК // Медицина: теория и практика. 2019. Т. 4, № S. С. 333–334.

[33] Ardatskaya MD, Bel’mer SV, Dobritsa VP, et al. Colon dysbacteriosis (dysbiosis): modern state of the problem, comprehensive diagnosis and treatment correction. Experimental & clinical gastroenterology. 2015;(5):13–50. (In Russ.)

[34] Ардатская М.Д., Бельмер С.В., Добрица В.П., и др. Дисбиоз (дисбактериоз) кишечника: современное состояние проблемы. Комплексная диагностика и лечебная коррекция // Экспериментальная и клиническая гастроэнтерология. 2015. № 5. С. 13–50.

[35] Krasakov IV, Litvinenko IV, Rodionov GG, Shantyr II, Svetkina EV. Evaluation of gut microbiota in Parkinson’s disease using gas chromatography with mass spectrometric detection. Annals of clinical and experimental neurology. 2018;12(4):23–29. (In Russ.) DOI: 10.25692/ACEN.2018.4.3

[36] Красаков И.В., Литвиненко И.В., Родионов Г.Г., Шантырь И.И., Светкина Е.В. Оценка микробиоты кишечника у пациентов с болезнью Паркинсона с помощью метода газовой хромато-масс-спектрометрии // Анналы клинической и экспериментальной неврологии. 2018. Т. 12, № 4. С. 23–29. DOI: 10.25692/ACEN.2018.4.3

[37] Rodionov GG, Shantyr II, Svetkina EV, Sar’yan ES, Vavilova TV. Evaluation of the wall intestinal microbiota of healthy people by gas chromatograthy — mass spectrometry method. Translational Medicine. 2017;4(6):34–42. (In Russ.) DOI: 10.18705/2311-4495-2017-4-6-34-42

[38] Родионов Г.Г., Шантырь И.И., Светкина Е.В., Сарьян Э.С., Вавилова Т.В. Оценка пристеночной микробиоты кишечника здоровых людей методом газовой хромато-масс-спектрометрии // Трансляционная медицина. 2017. Т. 4, № 6. С. 34–42. DOI: 10.18705/2311-4495-2017-4-6-34-42

[39] Platonova AG, Osipov GA, Boiko NB, Kirillova NV, Rodionov GG. The chromatography-mass spectrometry analysis of microbial fatty acids in human biological fluids and their clinical significance. Klin Lab Diagn. 2015;60(12):46–55. (In Russ.)

[40] Платонова А.Г., Осипов Г.А., Бойко Н.Б., Кириллова Н.В., Родионов Г.Г. Хромато-масс-спектрометрическое исследование микробных жирных кислот в биологических жидкостях человека и их клиническая значимость // Клиническая лабораторная диагностика. 2015. Т. 60, № 12. С. 46–55.

[41] Burmistrova AL, Filippova YuYu, Nokhrin DYu, Timofeeva AV. Society of environmental niche: oral cavity of the healthy children. Russian Journal of Infection and Immunity. 2018;8(1):54–60. (In Russ.) DOI: 10.15789/2220-7619-2018-1-54-60

[42] Бурмистрова А.Л., Филиппова Ю.Ю., Нохрин Д.Ю., Тимофеева А.В. Микробный социум экологической ниши: ротовая полость здоровых детей // Инфекция и иммунитет. 2018. Т. 8, № 1. С. 54–60. DOI: 10.15789/2220-7619-2018-1-54-60

[43] Kuklina EM, Smirnova EN, Nekrasova IV, Balashova TS. The role of B-lymphocytes in the presentation of autoantigens to CD4+-T-lymphocytes in autoimmune thyroiditis. Reports of the Academy of Sciences. 2015;464(4):508–511. (In Russ.) DOI: 10.7868/S0869565215280270

[44] Куклина Е.М., Смирнова Е.Н., Некрасова И.В., Балашова Т.С. Роль B-лимфоцитов в презентации аутоантигенов CD4+-Т-лимфоцитам при аутоиммунном тиреоидите // Доклады Академии наук. 2015. Т. 464, № 4. С. 508–511. DOI: 10.7868/S0869565215280270

[45] Shimizu J, Suzuki N. Associations of intestinal dysbiosis and T cell dysfunctions in patients with collagen related diseases, such as Behcet’s disease and relapsing polychondritis. Int J Clin Rheumatol. 2019;14(4):130–133.

[46] Shimizu J., Suzuki N. Associations of intestinal dysbiosis and T cell dysfunctions in patients with collagen related diseases, such as Behcet’s disease and relapsing polychondritis // Int. J. Clin. Rheumatol. 2019. Vol. 14, No. 4. P. 130–133.

[47] Shimizu J, Kubota T, Takada E, et al. Relative abundance of Megamonas hypermegale and Butyrivibrio species decreased in the intestine and its possible association with the T cell aberration by metabolite alteration in patients with Behcet’s disease (210 characters). Clin Rheumatol. 2019;38(5):1437–1445. DOI: 10.1007/s10067-018-04419-8

[48] Shimizu J., Kubota T., Takada E., et al. Relative abundance of Megamonas hypermegale and Butyrivibrio species decreased in the intestine and its possible association with the T cell aberration by metabolite alteration in patients with Behcet’s disease (210 characters) // Clin. Rheumatol. 2019. Vol. 38, No. 5. P. 1437–1445. DOI: 10.1007/s10067-018-04419-8

[49] Zdor VV. Correlation of hormonal and cytokines regulation in case of autoimmune thyroiditis. Clinical and experimental thyroidology. 2017;13(2):45–56. (In Russ.) DOI: 10.14341/ket2017245-56

[50] Здор В.В. Взаимосвязь гормональной и цитокиновой регуляции при аутоиммунном тиреоидите // Клиническая и экспериментальная тиреоидология. 2017. Т. 13, № 2. С. 45–56. DOI: 10.14341/ket2017245-56

[51] Troshina EA, Senyushkina ES. The value of central regulators of the immune response in the development of autoimmune thyroid diseases. Problems of Endocrinology. 2019;65(6):458–465. (In Russ.) DOI: 10.14341/probl10304

[52] Трошина Е.А., Сенюшкина Е.С. Вклад центральных регуляторов иммунного ответа в развитие заболеваний щитовидной железы // Проблемы эндокринологии. 2019. Т. 65, № 6. С. 458–465. DOI: 10.14341/probl10304

[53] Mizutani S, Yamada T, Yachida S. Significance of the gut microbiome in multistep colorectal carcinogenesis. Cancer Science. 2020;111(3):766–773. DOI: 10.1111/cas.14298

[54] Mizutani S., Yamada T., Yachida S. Significance of the gut microbiome in multistep colorectal carcinogenesis // Cancer Science. 2020. Vоl. 111, Nо. 3. P. 766–773. DOI: 10.1111/cas.14298

RIGHTS & PERMISSIONS

Raevskiy K.P., Solovev M.V.