IMMUNOCHEMICAL DIAGNOSIS OF CANCER. PROTOTYPING
S Kh Gantsev , A I Pukhalenko , A A Romaniukha , I A Chulina , A N Chulin , A B Poletaev
Physical and rehabilitation medicine, medical rehabilitation ›› 2019, Vol. 1 ›› Issue (3) : 58 -62.
IMMUNOCHEMICAL DIAGNOSIS OF CANCER. PROTOTYPING
A prototype of the method of immunochemical detection of different types of solid cancers (primary and recurrent) in the early stages was developed. According to the initial hypothesis, the sera of patients with malignant tumors of different localization and different histological nature, contain different sets of autoantibodies (auto-Ab) of IgG class to many cancer-associated antigens (CA-AG). The content of such auto-Ab differs in cancer patients and patients with non-malignant chronic diseases, which determines the difference in serum immunoreactivity profiles of cancer patients and patients with non-malignant diseases. Confirmation of this hypothesis opens up prospects for the creation of simple and cheap laboratory methods of mass preventive examination of the population for the early detection of different cancers. The confirmation of the hypothesis was obtained. Moreover, even with non-optimal sets of test antigens, with the help of solid-phase ELISA it was possible to achieve sensitivity of 71% and specificity of 68% in the differentiation of blood sera of cancer (lung, stomach, ovary, prostate) and non-cancer patients (chronic inflammatory diseases of the lungs, stomach, ovary, prostate) and nearly 90% in the differentiation of healthy individuals from cancer patients.
Oncology / solid tumors / laboratory diagnosis / enzyme-linked immunosorbent assay / autoantibodies
| [1] |
Дамиров М.М., Тютерева И.Н., Ганцев Ш.Х., Полетаева А.А., Полетаев А.Б., Юсупов А.С. Аутоиммунитет и рак. Новые подходы к ранней диагностике злокачественного роста. Креативная хирургия и онкология, 2011, 3, 89-93. |
| [2] |
Дрейпер Н. Р., Смит Г. Прикладной регрессионный анализ. М., Финансы и статистика, 2007. |
| [3] |
Полетаев А.Б. Физиологическая иммунология. М., Миклош, 2010 |
| [4] |
Полетаев А.Б. Иммунохимические маркеры опухолевого роста или как мы можем выявлять рак на ранних стадиях развития. Вестник МЕДСИ, 2011, 12, 19-24. |
| [5] |
Полетаев А.Б. Антитела к инсулиновым рецепторам как биомаркеры-предвестники сахарного диабета 2-го типа. Terra Medica, 2013, 71, 1, 22-26. |
| [6] |
Полетаев А.Б. Про пьяного и потерянные ключи. Клинич. Патофизиология, 2017, 23, 3, 3-13. |
| [7] |
Черезов А.Е. общая теория рака: тканевой подход. М.: изд-во МГУ, 1997. |
| [8] |
Backes C., Ludwig N., Leidinger P., et al. Immunogenicity of autoantigens. BMC Genomics 2011, 12:340 doi:10.1186/1471 -2164-12-340. |
| [9] |
Bergenfelz C., Medrek C., Ekström E. et al. Wnt5a Induces a Tolerogenic Phenotype of Macrophages in Sepsis and Breast Cancer Patients. J Immunol. 2012 [Epub ahead of print] PMID: 22547701 [PubMed - as supplied by publisher]. |
| [10] |
Cameron A.C., Trivedi P.K. Microeconometrics: Methods and Applications. Cambridge; New York: Cambridge University Press; 2005. |
| [11] |
Dvorak H.F. Tumors: wounds that do not heal. Similarities between tumor stroma generation and wound healing. N. Engl. J. Med. 1986, 315, 1650-1691. |
| [12] |
Meroni P.L., De Angelis V., Tedesco F. Future Trends. In: Autoantibodies, (Y. Shoenfeld, M. E. Gershwin, P. L. Meroni. Eds.), 823-826, Elsevier, B.V., 2007 |
| [13] |
Poletaev A. The Main Principles of Adaptive Immune System Function: Self-Recognition, Self-Interaction, and Self-Maintenance. In: Poletaev A. B., ed. Physiologic Autoimmunity and Preventive Medicine. Sharjah, Oak Park, Bussum: Bentham Science Publishers; 2013, 3-20. |
| [14] |
Xie C., Kim H. J., Haw J.G. et al. novel multiplex assay combining autoantibodies plus PsA has potential implications for classification of prostate cancer from non-malignant cases. J. Translational Medicine, 2011, 9, 43-53. |
Physical and rehabilitation medicine, medical rehabilitation
/
| 〈 |
|
〉 |
PDF
