Influence of the program for the accelerated recovery of postpartum women on the microviscosity of the erythrocyte membranes after cesarean section

Abstract

Background. This study aimed to analyze the microviscosity of membranes and the surface charge of erythrocytes and blood plasma proteins using fluorescent zones in pregnant women before and after cesarean section (CS) with traditional management of the perioperative period and use of an accelerated recovery program.

Materials and methods. The study included pregnant women without somatic or obstetric complications. They were divided into groups that received traditional management during the perioperative period (fasting for >8 h before surgery and administration of an antibiotic after clamping the umbilical cord) and used an accelerated recovery program. The relative microviscosity of the erythrocyte membranes was assessed using the lateral diffusion method with a hydrophobic pyrene probe (C16H10). The surface charge of erythrocytes and blood plasma proteins was determined using the fluorescent probe 8-anilino-1-naphthalene sulfonic acid.

Results. In postpartum women with an accelerated recovery program, the immersion of proteins in the lipid bilayer of the erythrocyte membrane decreased. The coefficient of pyrene eximerization in areas of protein–lipid contacts in the same group was higher and further increased both after anesthesia and after surgery. In the traditional management group, an increase in the eximerization coefficient was registered only after CS. In the areas of lipid–lipid contacts, the coefficient of pyrene eximerization in the accelerated recovery program group was significantly higher than that in the control group. Changes in the eximerization coefficient after anesthesia and surgery were insignificant. Moreover, the polarity of the microenvironment of both anular lipids and total lipids did not change.

Conclusions. An increase in the pyrene eximerization coefficient in patients on an accelerated recovery program indicates a decrease in the microviscosity of erythrocyte membranes and an increase in their fluidity, which may be due to structural rearrangements of cell membranes and leads to an increase in their functional activity. The components of the accelerated recovery program affect the microviscosity of erythrocyte membranes, particularly in the areas of protein–lipid contacts, which may positively affect microcirculation in the perioperative period, which plays a significant role in the genesis of postoperative complications.

Keywords

fluorescent probe ANS (8-anilino-1-naphthalene sulfonic acid) / blood plasma proteins / cesarean section / membrane microviscosity / accelerated recovery program / pyrene / kinetic parameters / fluorescent probes / fast track / accelerated recovery program / perioperative period

References

[1] Gumeniuk EG, Kormakova TL, Karpechenko AV, Marabyan LM. Repeated Caesarean section in modern obstetrics. Are there decreasing reserves? RUDN Journal of Medicine. 2015;(3):117–122.

[2] Гуменюк Е.Г., Кормакова Т.Л., Карпеченко А.В., Марабян Л.М. Повторная операция кесарева сечения в современном акушерстве. Есть ли резервы снижения? // Вестник РУДН. Серия: Медицина. 2015. № 3. С. 117–122.

[3] Petrikovsky B, Ruggiero R, Medvedeva P, Kozlov I. Cesarean Section in 21st Century. Voprosy ginekologii, akusherstva i perinatologii. 2015;14(1):78–82.

[4] Петриковский Б., Руггиеро Р., Медведева П., Козлов И. Кесарево сечение в 21 веке // Вопросы гинекологии, акушерства и перинатологии. 2015. Т. 14, № 1. С. 78–82.

[5] Medzhidova DR, Shifman EM, Cherkesova AU, Cherkesova DU. Lipid peroxidation and oxidative damage of maternal plasma and erythrocyte membrane lipids in abdominal delivery with the use of an enhanced rapid recovery programme. Voprosy ginekologii, akusherstva i perinatologii. 2020;19(3):57–62. doi: 10.20953/1726-1678-2020-3-57-62

[6] Меджидова Д.Р., Шифман Е.М., Черкесова А.У., Черкесова Д.У. Перекисное окисление липидов и окисленность белков плазмы крови и мембран эритроцитов матери при абдоминальном родоразрешении с использованием программы ускоренного выздоровления // Вопросы гинекологии, акушерства и перинатологии, 2020. Т. 19, № 3. С. 57–62. doi: 10.20953/1726-1678-2020-3-57-62

[7] Vladimirov YuA, Dobretsov GE. Fluorescent probes in the study of biological membranes. Moscow: Nauka; 1980. (In Russ).

[8] Владимиров Ю.А., Добрецов Г.Е. Флуоресцентные зонды в исследовании биологических мембран. Москва : Наука, 1980.

[9] Kamkin AG, Kiseleva IS. Physiology and molecular biology of cell membranes. Moscow: Akademiya; 2008. 592 p. (In Russ).

[10] Камкин А.Г., Киселева И.С. Физиология и молекулярная биология мембран клеток. Москва : Академия, 2008. 592 с.

[11] Klenova NA, Klenov RO. The structure, metabolism and functional activity of human erythrocytes in normal and pathological conditions. Samara: Samarskii universitet; 2009. 116 p. (In Russ).

[12] Кленова Н.А., Кленов Р.О. Строение, метаболизм и функциональная активность эритроцитов человека в норме и патологии. Самара : Самарский университет, 2009. 116 с.

[13] Chang NT. Research of the structural condition of membranes erythrocytes of advanced ages sick of the ischemic heart trouble. Fundamental research. 2012;(2):97–103.

[14] Чанг Н.Т. Исследование структурного состояния мембран эритроцитов больных ишемической болезнью сердца старшего возраста // Фундаментальные исследования. 2012. № 2. С. 97–103.

[15] Dobretsov GE. Fluorescent probes in the study of cells, membranes, and lipoproteins. Moscow: Nauka; 1989. 227 p. (In Russ).

[16] Добрецов Г.Е. Флуоресцентные зонды в исследовании клеток, мембран, липопротеинов. Москва : Наука, 1989. 227 с.

[17] Dontsov VI, Mal’tseva IV, Chernilevskii VE. Fluorescent probes in the study of intracellular changes in aging: changes in the microviscosity of cell membranes. In: Chernilevskii VE, Krut’ko VN, Kudashov AA, editors. Doklady Moskovskogo obshchestva ispytatelei prirody. Sekciya Gerontologiya. Moscow; 2013;55:73–79. (In Russ).

[18] Донцов В.И., Мальцева И.В. Чернилевский В.Е. Флуоресцентные зонды в изучении внутриклеточных изменений при старении: изменения микровязкости мембран клеток. В кн.: Доклады МОИП. Секция Геронтология. Москва, 2013. Т. 55. С. 73–79.

[19] Ivanova SV, Kirpichenok PN. The use of fluorescent methods in medicine. Meditsinskie novosti. 2008;12:56–61. (In Russ).

[20] Иванова С.В., Кирпичёнок Л.Н. Использование флуоресцентных методов в медицине // Медицинские новости. 2008. Т. 12. С. 56–61.

[21] Panin LE, Mokrushnikov PV, Kunitsyn VG, Panin VE, Zaitsev BN. Fundamentals of multilevel mesomechanics of nanostructural transitions in erythrocyte membranes and their destruction in interaction with stress hormones. Physical Mesomechanics. 2011;14(1):5–17.

[22] Панин Л.Е., Мокрушников П.В., Куницын В.Г., Панин В.Е., Зайцев Б.Н. Основы многоуровневой мезомеханики наноструктурных переходов в мембранах эритроцитов и их разрушения при взаимодействии с гормонами стресса // Физическая мезомеханика. 2011. Т. 14, № 1. С. 5–17.

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