Lipopolysaccharide-induced model of inflammation in cells culture

Abstract

Inflammation is a non-specific process that underlies the pathogenesis of many diseases, including COVID-19. The study of the mechanisms and molecular pathways of this phenomenon is carried out using various models, including in vitro investigations on cell cultures. The most accessible model for reproduction is the lipopolysaccharide-induced one.

This review analyzes the scientific papers in which the model was represented in vitro. It was found that inflammation caused by lipopolysaccharide is realized by activating classical signaling pathways (nuclear factor "kappa-bi", mitogen-activated protein kinase, etc.), changes of expression of circular and non-coding RNAs, etc. Evaluation of the working model of inflammation was carried out by many parameters, the most important of which was the concentration of pro-inflammatory cytokines (IL-6, IL-8, IL-1β, TNF-α). Inflammation through stimulation with lipopolysaccharide was reproduced in cultures of different cells, however, it was most easily reproduced in the culture of monocytes and macrophages, which is explained by their conversion to the M1 phenotype. The co-cultivation of different cells made it possible to study the mechanisms of the pathological process in the model of inflammation more fully.

Keywords

inflammation / lipopolysaccharide-induced model / cell culture / cytokines / COVID-19

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