The morphology and chemistry of a rat spinal cord after thoracal segmentectomy and transplantation of polymeric collagen neuromatrix “Spherogel - E”™ containing incorporated ensheathing neuroepithelial cells

Abstract

Using classical histological methods and immunocytochemical reaction on GAP43 and NFSOO we studied the process of tissue reorganizations and neurochimical changes accompanying the scar formation in rat spinal cord after thoracal segmentectomy and subsequenttransplantation of olfactory ensheathing cells, incorporated in neuromatrix «Sperogel». In spinal cord of control rats formation on gliomesodermal capsulus was the result of classical histoproliferative reaction. The morphology of scar tissue in experimental rats differed markedly from control group, its formations began at early stages and were associated with proliferation of both connective tissue and astrocites, and macrophages infiltration. As result the new tissue included a lot of capillaries and bundles of nerve fibers with GAP43 and NF-300 immunopositivity. We conclude that the better tissue repair, their revascularization and nerve regeneration, were the result of two main factors: 1 - modulating role of neuromatrix; 2 - metabolic and neurotrophic activity of trasplantated olfactory ensheathing cells.

Keywords

spinal cord / ensheathing neuroepithelial cells / segmentectomy / scar / immunohistochemical study

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