Analysis of laboratory screening for cord blood banking
O. V. Tyumina , G. I. Gusarova , V. V. Pavlov , M. N. Zharkov , S. E. Volchkov , A. N. Toropovsky , N. N. Krasnova , I. A. Nizhegorodtseva , V. A. Rossiev , V. G. Savchenko
Genes & Cells ›› : 72 -73.
Analysis of laboratory screening for cord blood banking
The purpose of the study is to analyze laboratory screening for the presence of infectious agents in the procurement of cord blood for public use. We examined 586 donor PC samples by immuno-enzyme analysis in the first 24 hours after delivery for the presence of the following serological markers: NiѴ-1 and-2 Ag/Ab, Anti-HBcor, HBS-Ag, Anti-HCV, Anti-CMV, Anti-Toxoplasma gondii, Anti-HSV-1 and -2, RW, bacteriological examination of PC samples was also carried out. It was found that 73 [12.5%] PC samples were disposed of due to infectious danger. Of these, a positive analysis for HBs-Ag was detected in 3 samples [0.5%]; Anti-HCV - in 5 samples [0.9%]; RW — in 6 samples [1%]; Anti-HBcor - in 59 samples [1O%]. The percentage of germination of microbial contaminants was 0.1%. Thus, in order to reduce the percentage of PC samples disposal, an Anti-HBcor analysis should be included in the mandatory laboratory screening of female PC donors. The use of closed methods of blood sampling and processing, as well as ready-made universal media, allows virtually eliminating the possibility of contamination of PC samples with aerial flora.
cord blood / contamination / laboratory screening
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Приказ М3 РФ №364 от 14.09.01 «Об утверждении порядка медицинского обследования донора крови и её компонентов». |
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Приказ М3 РФ №325 от 25.07.03 «О развитии клеточных технологий в Российской Федерации» |
Eco-Vector
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