Quantitative accounting of CD34+ hematopoietic stem cells in whole cord blood

Abstract

Umbilical cord blood is an alternative source of hematopoietic stem cells to bone marrow and mobilized peripheral blood [HSC]. The population of CD34" cells in umbilical cord blood is small, which creates certain difficulties in carrying out quantitative accounting of HSC. This article discusses some practical aspects of determining CD34" HSC in whole cord blood. A preliminary assessment of the amount of HSC in the samples before the start of preparation for long-term storage may become an additional selection criterion when receiving cord blood in non-commercial anonymous banks.

Keywords

cord blood / CD34+ hematopoietic stem cells / flow cytometry

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