CXCR4 expression and CD34+ hematopoietic stem cells mobilization efficacy

Abstract

CXCR4 is a chemokine receptor which is essential for targeted migration of hematopoietic stem cells (HSC). CXCR4 is known to be an indirect target for G-CSF stimulation. The aim of this study was to evaluate the level ofCXCR4 expression on the surface of HSC, collected from apheresis products of patients with different responses to G- CSF-stimulation. Cord blood, normal bone marrow and primed bone marrow samples were analyzed to be compared with apheresis product. It was demonstrated that mean fluorescence intensity of CXCR4 indicating the density of CXCR4 expression on HSC surface is diminished after G-CSF stimulation towards normal bone marrow ? primed bone marrow ? apheresis product from mobilizable patients and demonstrates negative correlation with mobilization efficacy. CXCR4 expression on the surface of HSC from non-mobilizable patients does not differ from other native sources, such as normal bone marrow and cord blood, that points out the intrinsic unresponsiveness to G- CSF treatment or other kinetics of mobilization in poor-mobilizable patients.

Keywords

CXCR4 / CD34+ hematopoietic stem cells / mobilization

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