Continuity of cytogenetic characteristics in passages of mice embryonic germ cell line G1

Abstract

The comparative analysis of cytogenetic characteristics in populations of cells of different passages of mice embryonic germ cell line G1 received from sexual puff of 12,5 days of embryo of the mouse of line BALB/c was carried out. It was revealed, that despite of the expressed heterogeneity of cells on number of chromosomes, Robertsonian translocation, some line specific karyotype features were characteristic for all investigated cell populations. It was, in particular, the closest of the chromosome numbers to penta- and hexaploidies, and also the presence of translocation t(6;12). The possible connections between revealing karyotype traits and the mechanisms of maintenance of cell polypotency and proliferation in cultural condition were discussed.

Keywords

embryonic germ cell / Robertsonian translocation

References

[1] Park J.H., Kim S.J., Lee J.B. et al. Establishment of a human embryonic germ cell line and comparison with mouse and human embryonic stem cells. Mol. Cells 2004; 17(2): 309-15.

[2] Mitalipov S.M., Mitallipova M.M., Ivanov V.I. The effect of the duration of culturing on the pluripotency of mouse embryonic stem (ES) cells in vitro and in vivo. Ontogenez 1994; 25(6): 19-27.

[3] Волгарева Г.М. Хромосомные маркеры мышиных гибридом. Журн. микробиол. эпидемиол. иммунобиол. 1986; 18(2): 69-73.

[4] Sugawara A., Goto K., Sotomaru Y. et al. Current status of chromosomal abnormalities in mouse embryonic stem cell lines used in Japan. Comparative Medicine 2006; 56(1): 33-6.

[5] Лукаш Л.Л., Яцишина А.П., Підпала О.В. и др. Одержання нових ліній стовбурових клітин миші і вивчення впливу мікрооточення на їхню каріотипічну мінливість in vitro. Физиология и биохимия культурных растений 2006; 38(2): 144-52.

[6] Яцишина А.П., Підпала О.В., Кочубей Т.П., Лукаш Л.Л. Спонтанна каріотипічна еволюція клітин in vitro. Фактори експериментальної еволюції організмів 2004; Київ: КВІЦ: 88-92.

[7] Cowell J.K A photographic representation of the variability in the G-banded structure of the chromosomes in the mouse karyotype. A guide to the identification of the individual chromosomes. Chromosoma 1984; 89(4): 294-320.

[8] Raz R., Lee C.-K., Cannizzaro L.A. et al. Essential role of STAT3 for embyonic stem cell pluripotency. Proc. Natl. Acad. Sci. USA 1999; 96(6): 2846-51.

[9] Глазко Т.Т. Кариотипические особенности ряда клональных линий межвидовой гибридомы мышь-норка. Цитология 1988; 30(5): 597-605.

[10] Silva S., Kovalchuk A.L., Kim J.S. et al. BCL2 Accelerates inflammation- induced balb/c plasmacytomas and promotes novel tumors with coexisting t(12;15) and t(6;15) translocations. Cancer Research 2003; 63(24): 8656-63.

[11] Ivanova N., Dobrin R., Lu R. et al. Dissecting self-renewal in stem cells with RNA interference. Nature 2006; 442(7102): 533-8.

RIGHTS & PERMISSIONS

Eco-Vector