The structure, functions and viability of the umbilical cord blood nuclear cells after cryoconservation
L A Babiychuk , V I Grishchenko , P M Zubov , O L Zubova , V V Ryazantsev , L V Babiychuk , O V Kudokotseva , S A Lyubchich , L A Babyichuk , V I Grishchenko , P M Zubov , O L Zubova , V V Ryazantsev , L V Babyichuk , O V Kudokovtseva , S A Lyubchich
Genes & Cells ›› 2010, Vol. 5 ›› Issue (3) : 77 -81.
The structure, functions and viability of the umbilical cord blood nuclear cells after cryoconservation
New efficient methods for separation and cryopreservation of nucleated cells from umbilical cord blood, which allow you to save a quantitative and qualitative composition of nucleated cells (including hematopoietic stem] after discharge, and demonstrate a high level of preservation of structural and functional properties and viability of CD45+ and CD34+-cells after cryopreservation.
umbilical cord blood hematopoietic stem cells / cryopreservation / DMSO / lipid asymmetry / viability
| [1] |
Long G.D., Laughlin М., Madan В. et al. Unrelated umbilical cord blood transplantation in adult patients. Biol. Blood Marrow Transplant. 2003; 9(12): 772-80. |
| [2] |
Gluckman E. Broxmeyer H.A., Auerbach A.D. et al. Hematopoietic reconstitution in a patient with Fanconi's anemia by means of umbilical-cord blood from an HLA-identical sibling. N. Engl. J. Med. 1Э8Э; 321(17): 1174-8. |
| [3] |
Абдулкадыров K.M., Романенко H.A. Заготовка плацентарной крови. Особенности ее клеточного состава и гемопоэтическога потенциала. Трансфузиология 2003; 4(1): 15-33. |
| [4] |
Cornetta К., Laughlin М., Carter S. et al. Umbilical cord blood transplantation in adults: results of the prospective Cord Blood Transplantation (COBLT1. Biol. Blood Marrow Transplant. 2005; 11(21: 149-60. |
| [5] |
Владимирская Е.Б., Майорова О.А., Румянцев С.А., Румянцева А.Г. Биологические основы и перспективы терапии стволовыми клетками. 2005. М.: Медпрактика: 391. |
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