A novel type of a polytetrafluorethylene-based tissue-engineered construction with nanopattered multifunctional biocompatible nonresorbed coating

A S Grigor'yan; E V Kiseleva s; D V Shtanskiy; M R Filonov; T K Khamraev; A K Toporkova; A V Gastiev; Sh Farkashdi; A S Grigoryan; E V Kiseleva; M P Filonov; D V Shtanskij; Т К Khamraev; A K Toporkova; A B Gastiev; Sh Farkashdi

doi:10.23868/gc121774

Genes & Cells ›› 2010, Vol. 5 ›› Issue (3) : 71 -76. DOI: 10.23868/gc121774

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A novel type of a polytetrafluorethylene-based tissue-engineered construction with nanopattered multifunctional biocompatible nonresorbed coating

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Central Research Institute of Stomatology and Maxillo-Facial Surgery of Federal Agency for High-Technology Medical Care, Moscow

Russian Academy of Sciences [RAS] Koltzov Institute of Developmental Biology, Moscow

The Federal State Educational Institution of the Higher Professional Education «National University of Science and Technology «MISiS», Moscow

Russian Medical Academy of Postgraduate Education, Moscow

Peoples' Friendship University of Russia, Moscow

Central Research Institute of Stomatology and Maxillo-Facial Surgery of Federal Agency for High-Technology Medical Care, Moscow

Russian Academy of Sciences [RAS] Koltzov Institute of Developmental Biology, Moscow

The Federal State Educational Institution of the Higher Professional Education «National University of Science and Technology «MISiS», Moscow

Central Research Institute of Stomatology and Maxillo-Facial Surgery of Federal Agency for High-Technology Medical Care, Moscow

Russian Medical Academy of Postgraduate Education, Moscow

Peoples' Friendship University of Russia, Moscow

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Abstract

Here we preset a method for tissue engineering construct based on highly porous PTFE with nanostructured multifunctional biocompatible non-degradable coating (MBNC) with composition of Ti-Ca-P-C-O-N. It is shown that scaffolds composed of PTFE with MBNC are suitable for adipose-derived multipotent stromal cells CADMSJ active adhesion and proliferation. Under osteogenic stimulation ADMS cells were differentiated to osteogenic lineage, which was confirmed by positive immunohistochemical reaction for osteopontin and osteonectin. Using the experimental model of critical calvarial defects in rabbits we investigated the influence of tissue engineering construct [membrane from highly porous PTFE with MBNC carrying ADMS cells) transplantation on bone defect regeneration. It was shown that under tissue engineering construct the bone defect was completely closed with newly formed bone tissue in 3 and В months after transplantation.

Keywords

tissue engineering construct / functional coverage / multipotent mesenchymal stromal cells of adipose tissue / bone regeneration / cranioplasty

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A S Grigor'yan, E V Kiseleva s, D V Shtanskiy, M R Filonov, T K Khamraev, A K Toporkova, A V Gastiev, Sh Farkashdi, A S Grigoryan, E V Kiseleva, M P Filonov, D V Shtanskij, Т К Khamraev, A K Toporkova, A B Gastiev, Sh Farkashdi. A novel type of a polytetrafluorethylene-based tissue-engineered construction with nanopattered multifunctional biocompatible nonresorbed coating. Genes & Cells, 2010, 5(3): 71-76 DOI:10.23868/gc121774

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