Effect of Pluronic Р85 on proliferation and osteogenic differentiation of human mesenchymal stem cells in vitro

NL Blatt; M E Yalvach; A K Shafigullina; I I Salafutdinov; A L Kiyasov; R F Masgutov; YuG Shtyrlin; A V Kabanov; A A Rizvanov; N L Blatt; ME Yalvac; A K Shafigullina; I I Salafutdinov; A P Kiyasov; R E Masgutov; Y G Shtirlin; A V Kabanov; A A Rizvanov

doi:10.23868/gc121773

Genes & Cells ›› 2010, Vol. 5 ›› Issue (3) : 66 -70. DOI: 10.23868/gc121773

Articles

other

Effect of Pluronic Р85 on proliferation and osteogenic differentiation of human mesenchymal stem cells in vitro

Author information +

Kazan Federal University, Kazan

Yeditepe University, Istanbul, Turkey

Kazan State Medical University, Kazan

Kazan Federal University, KazanKazan State Medical University, Kazan

Medical Center of University of Nebraska, Omaha, USALomonosov Moscow State University, Moscow

Kazan Federal University, KazanYeditepe University, Istanbul, TurkeyKazan State Medical University, Kazan

Kazan Federal University, Kazan

Yeditepe University, Istanbul, Turkey

Kazan State Medical University, Kazan

Kazan Federal University, Kazan

Kazan State Medical University, Kazan

Kazan Federal University, KazanKazan State Medical University, Kazan

Kazan Federal University, Kazan

Medical Center of University of Nebraska, Omaha, USALomonosov Moscow State University, Moscow

Kazan Federal University, KazanYeditepe University, Istanbul, TurkeyKazan State Medical University, Kazan

Show less

History +

PDF

Abstract

We investigated affect of non-ionic block copolymer (Pluronic) P85 on in vitro proliferation and osteogenic differentiation of human mesenchymal stem cells (MSCs). We demonstrated that Pluronic P85 at final concentration 0,1-1% was cytotoxic to MSCs. Addition of Pluronic P85 at final concentration 0,001-0,01% increased proliferation of MSCs. Pre-incubation of MSCs with Pluronic P85 increased osteogenic differentiation efficiency of cells.

Keywords

Pluronic / mesenchymal stem cells / proliferation / osteogenesis / differentiation

Cite this article

Download citation ▾

NL Blatt, M E Yalvach, A K Shafigullina, I I Salafutdinov, A L Kiyasov, R F Masgutov, YuG Shtyrlin, A V Kabanov, A A Rizvanov, N L Blatt, ME Yalvac, A K Shafigullina, I I Salafutdinov, A P Kiyasov, R E Masgutov, Y G Shtirlin, A V Kabanov, A A Rizvanov. Effect of Pluronic Р85 on proliferation and osteogenic differentiation of human mesenchymal stem cells in vitro. Genes & Cells, 2010, 5(3): 66-70 DOI:10.23868/gc121773

登录浏览全文

4963

注册一个新账户忘记密码

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	Batrakova E.V., Kabanov A.V. Pluronic block copolymers: evolution of drug delivery concept from inert nanocarriers to biological response modifiers. J. Control. Release. 2008; 130(2): 98-106.

[2]	Batrakova E.V., Li S., Alakhov V.Y. et al. Sensitization of cells overexpressing multidrug-resistant proteins by pluronic P85. Pharm Res. 2003; 20(10): 1581-90.

[3]	Batrakova E.V., Li S., Brynskikh A.M. et al. Effects of pluronic and doxorubicin on drug uptake, cellular metabolism, apoptosis and tumor inhibition in animal models of MDR cancers. J Control Release 2010; 143(3): 290-301.

[4]	Gaymalov Z.Z., Yang Z., V.M. Pisarev, et al., The effect of the nonionic block copolymer pluronic P85 on gene expression in mouse muscle and antigen-presenting cells. Biomaterials 2009; 30(B): 1232-45.

[5]	Yalvac M.E., Ramazanoglu M., Rizvanov A.A. et al. Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis. Pharmacogenomics J. 2009; 10(2): 105-13.

[6]	B. Lisignoli G., Cristino S., Piacentini A. et al. Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown n a three-dimensional hyaluronan based scaffold. Biomaterials 2005; 26(28): 5677-86.

[7]	Alliot-Licht В., Bluteau G., Magne D. et al. Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures. Cell Tissue Res. 2005; 321(3): 391-400.

[8]	Yalvac M.E., Ramazanoglu M., Gumru O.Z. et al. Comparison and optimisation of transfection of human dental follicle cells, a novel source of stem cells, with different chemical methods and electro-poration. Neurochem Res. 2009; 34(7): 1272-7.

[9]	Yalvac M.E., Ramazanoglu M., Rizvanov A.A. et al. Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis. Pharmacogenomics J. 2010; 10(2): 105-13.

[10]	Hoemann CD., El-Gabalawy H., McKee M.D. In vitro osteogenesis assays: influence of the primary cell source on alkaline phosphatase activity and mineralization. Pathol Biol (Paris). 2009; 57(4): 318-23.