Comparative study of an activity of rat spinal ganglion cells and PC12 cells on the surfacesmodified with bioadhesive polymers

Abstract

We studied the adsorption of bioadhesive polymers(polyornithine, gelatin, laminin) on polystyrene surfaceby the use of dynamic light scattering. The contributionof biopolymers to resulting zeta potential of the modifiedsurface was assessed. PC12 cells do not exhibit selectiveadhesion in the presence of foetal bovine serum. Polystyrenewith adsorbed polyornithine promotes primary adhesionof PC12 cells cultured in serum-free medium with nervegrowth factor. Subsequently adsorbed laminin inducesspreading and differentiation of the cells into neuronaldirection. Primary neurons isolated from rat spinal ganglionadhere preferentially on the polyornithine-modified surface.On the polyornithine-laminin surface neurons intensivelyform neuritis that correlates with proliferation of glialcells positive for S100 protein. The results show thatPC12 cells and primary neurons exhibit similar response tosurface material with the latter cells being more sensitiveto this factor. Isolated cell culture can be used to study therelationship between neurite outgrowth and Schwann cellsproliferation on different biomaterials.

Keywords

cell models / peripheral nerve / regeneration / biomaterials / bioadhesive polymers

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