An in vivo study of pha matrices of different chemical composition: tissue reaction and biodegradation

Abstract

The study addresses consequences of subcutaneousimplantation of film matrices prepared from different PHAsto laboratory animals. No negative effects of subcutaneousimplantation of PHA matrices on physiological and biochemicalcharacteristics of the animals were determined. Independentlyof the matrices composition and duration of the contact withthe internal environment of the organism we did not observeany deviations in the behavior of animals, their growth anddevelopment, as well as blood functions. Response of thetissues to PHA matrices was comparable with the response topolylactide, but substantially less expressed at the earlier timeperiods after implantation. Tissues response to implantation ofPHA of all types is characterized by short-term (up to 2 weeks)post-traumatic inflammation with formation of fibrous capsulesby 30th-60th days with the thickness less than 100 microns,which get thinner down to 40-60 microns by 180th day as theresult of involution. No differences in response of tissues andthe whole organism were observed for the matrices producedfrom the homopolymer of 3-hydroxybutyric acid (P3HB),copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids(P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianicacids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoateacids (P3HB/3HH). Macrophages and foreign-body giant cellsactively participate in the response of the tissues to PHAs. Inthe studied conditions matrices from the copolymers containing3-hydroxyhexanoate and 4 hydroxybutyrate were determined asmore actively degraded PHA. The next less degraded matriceswere matrices from the copolymer of P3HB/3HV and the mostresistant were P3HB matrices. The slower degradation of PHAmatrices was accompanied by delayed development of giantcellsresponse. The studied PHA matrices can be placed in thefollowing range by their degradation: P3HB/3HH - P3HB/4HB -P3HB/HV - P3HB.

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