Obtaining and characteristics of human induced pluripotent stem cells from skin fibroblastsof patients with neurodegenerative diseases

E D Nekrasov; O S Lebedeva; I V Chestkov; M A Syusina; E Yu Fedotova; M A Lagarkova; S L Kiselev; I A Grivennikov; S N Illarioshkin

doi:10.23868/gc121688

Genes & Cells ›› 2011, Vol. 6 ›› Issue (4) : 82 -88. DOI: 10.23868/gc121688

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Obtaining and characteristics of human induced pluripotent stem cells from skin fibroblastsof patients with neurodegenerative diseases

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N.I. Vavilov Institute of General Genetics, RAS, Moscow

N.I. Vavilov Institute of General Genetics, RAS, MoscowMolecular Genetics Institute, RAS, Moscow

Research Neurology Center, RAMS, Moscow

N.I. Vavilov Institute of General Genetics, RAS, MoscowNational Research Institute «Kurchatov Institute», Moscow

Molecular Genetics Institute, RAS, Moscow

N.I. Vavilov Institute of General Genetics, RAS, Moscow

N.I. Vavilov Institute of General Genetics, RAS, MoscowMolecular Genetics Institute, RAS, Moscow

N.I. Vavilov Institute of General Genetics, RAS, Moscow

Research Neurology Center, RAMS, Moscow

N.I. Vavilov Institute of General Genetics, RAS, Moscow

N.I. Vavilov Institute of General Genetics, RAS, MoscowNational Research Institute «Kurchatov Institute», Moscow

Molecular Genetics Institute, RAS, Moscow

Research Neurology Center, RAMS, Moscow

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Abstract

Induced pluripotent stem cells (iPSCs) are analogousto embryonic stem cells in their properties, and might bederived from adult somatic cells. There exist two mainapproaches to practical application of iPSCs technologies -they are regenerative medicine and human diseases modeling.Nowadays the process of iPSCs obtaining is being investigatedin many world laboratories, but there are no standards in theiPSC obtaining technology. This work was aimed to develop asimple, cheap and practical protocol for obtaining iPSCs andto establish a collection of iPCS lines derived from cells ofpatients with a Parkinson disease to make a cell model of thisdisease. The work resulted in a development of the protocolto produce iPSCs based on a lentiviral delivery of transgenesinto cells. iPCS lines from 3 patients with inherited Parkinsondisease forms have been obtained.

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E D Nekrasov, O S Lebedeva, I V Chestkov, M A Syusina, E Yu Fedotova, M A Lagarkova, S L Kiselev, I A Grivennikov, S N Illarioshkin. Obtaining and characteristics of human induced pluripotent stem cells from skin fibroblastsof patients with neurodegenerative diseases. Genes & Cells, 2011, 6(4): 82-88 DOI:10.23868/gc121688

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