Cryopreservation human placental tissue as source of hematopoietic and mesenchymal stem cells

Abstract

In this paper we have show the possibility of cryopreservationof placental tissue for the reason to obtain viable hematopoieticprogenitor cells and multipotent mesenchymal stromal cells.It is established, that the relative content of the cells of CD34+/SSClow/CD45low cells significantly higher in the cryopreservedplacental tissue. Also we first described the differencesexpression of CD90 and CD31on the CD34+/CD45low/SSClowcells of umbilical cord blood and placenta. Clonal analysis ofcells revealed the presence in cryopreserved placental tissuesof precursors of granulocytes, monocytes and erythrocytesthat forming colonies of granulocyte-monocyte, monocytes,granulocytes and erythrocytes. Also it was obtained culturesof stromal cells from cryopreserved placental tissues withimmunophenotype CD90+/CD73+/CD105+/HLA-ABClow/CD45-/CD34-/CD133-/CD14- and adipogenic and osteogenic potentialin vitro.

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