Personalized cell-based therapy in ophthalmology: the cytokine profile of autologous cell products

Abstract

The content of the main pro- and anti- inflammatory cytokinesand growth factors in autologous cell products, designedfor personalized cell-based therapy in corneal endotheliumlesions was studied. It was established that cell productcontains substances that mediate the effectiveness of celltherapy. The influence of processing conditions on the concentrationof cytokines and growth factors in cell products isclarified. It is established that cytokine profile of the cell productis composed of pre-existing substances in freshly bloodserum and plasma (TGF-β1, TGF-β2, b-FGF, PDGF-AB, VEGF,IL-4, IF-γ) and those concentration of which increased as aresult of processing the cell product - incubation for 4 hoursat 37°C (IL-8) and stimulation with polyA:U (IL-1, 6, IF-α andTNF-α). The evaluation of cytokine profile of cell preparationreveals implementation mechanisms of therapeutic effect ofpersonalized cell-based therapy.

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