Comparative investigation of polyhydroxyalkanoate scaffolds with various chemical compositions

Abstract

The authors have constructed and characterized a seriesof membranes based on resorbable polyhydroxyalkanoates ofdifferent compositions. Five PHA types have been studied: ahomopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyricand 4-hydroxybutyric acids, 3-hydroxybutyricand 3-hydroxyvaleric acids, 3-hydroxybutyric and 3-hydroxyhexanoicacids. Scanning electron microscopy and atomicforcemicroscopy were used to examine the microstructureof membrane surfaces, showing that membranes based onthe copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoatehad the roughest surface, while membranes based on the copolymerof 3-hydroxybutyrate and 3-hydroxyvalerate had thesmoothest surface. The contact angle for water in air wassmaller and hydrophilic properties better in the copolymermembranes than in the membranes based on the high-crystallinityhomopolymer of 3-hydroxybutyric acid. The culture ofmouse fibroblast cell line NIH 3Т3 was used to test PHAbasedmembranes; results of fluorescent probes of DNA DAPIand the MTT assay show that membranes based on studiedPHAs are not cytotoxic on direct contact with cells and arehighly biocompatible; their adhesive properties and ability tomaintain fibroblast proliferation are similar to those of polystyreneand better than those of polylactic acid membranes.

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