Experimental treatment with allogeneic multipotent mesenchymal stromal cells pulmonary emphysema in rats

Abstract

The aim of the study was to asses morphologic andmorphometric lung tissue changes and peritoneal macrophagesactivity (MA) after allogeneic multipotent mesenchymalstromal cells (MMSC) systemic transplantation in acuteelastase model of pulmonary emphysema(PE) in rats.Methods. Forty 3-months old Wistar rats were randomizedinto 4 groups. Control group (1 group) was injectedintratracheally 0,4 ml of normal saline, other animals (2-4groups) received one intratracheal injection of 20 units (U)porcine pancreatic elastase in 0,4 ml of saline. Next day (3group) and 7 day (4 group) rats were intravenously injected2106 autologous MMSC in 0,5 ml of saline. 2 group wasused as emphysema control. Before euthanizing at the 21stday rats were undergone peritoneal lavage with analysis ofhemi-luminescent macrophages activity in the obtained fluid.Results. The lungs of 2-4 groups had various degreesof PE. The imean linear intercept in group 2 experimentalemphysema increased by 231% versus the control group. Thetransplantation of MSCs in a day after elastase decreasedmean linear intercept by an average of 50.3% compared to thecontrol of experimental emphysema. In animals MMSC injectedat the 7 th day of study, this sizes decreased more greater -by 64,5%, but was higher by 40.7% compared to the firstcontrol group. Another quantitative measure of PE alveolarindex was significantly increased by 149% in group 2.The transplantation of MMSC at 1-st and 7-th day of experimentleaded to alveolar index decreasing, respectively on 163.4and 237% . The peak Index of hemi-luminescent macrophagesactivity (Mv/106 cells) has made 28,8+1,1 (1 group),57,3+1,3 (2 group), 35,8+1,6 (3 group), 31,9+1,9(4 group).Conclusions. Our study confirmed the possibility ofregenerative lung tissue effect of autologous MMSCintravenous injected in experimental rat models of PE

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