Influence of recombinant histone H1.3 on the efficiency of lentiviral transduction of human cells in vitro

Abstract

Lentiviral vectors are widely used in genetic modificationof human and animal cells (lentiviral transduction) to enhancetheir therapeutic potential by expression of recombinantprotective and trophic factors. Genetic modification of cells invitro or ex vivo achieves the specificity of viral transduction,as modified are just cells that have been manipulated inthe laboratory. In addition, the introduction of geneticallymodified cells, but not pure virus, helps to avoid introductionof viral particles into the body of the recipient. This approachallows us to control the expression of therapeutic genes, theimmunogenicity of viral vectors and viral transduction. Todate, different approaches are used to improve the lentiviraltransduction (polycations, protamine sulfate, etc.), but thesemethods suffer from limited efficacy or high toxicity. For thefirst time we demonstrated that the recombinant histoneN1.3 increases the efficiency of lentiviral transduction bymore than 2 times and has no toxic effect on target cells ina wide range of concentrations studied.

Keywords

histone H1.3 / lentiviruses / retroviruses / transduction / cytotoxicity

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