Survival and differentiation of endogenous Schwann cells migrating into spinal cordunder the influence of neurotrophic factors

Abstract

Schwann cells are a major figure in the process ofregeneration in the peripheral nervous system. They migrateinto the injury region of spinal cord, which are involved inremyelination and are regarded as the source of numerousmolecular signals that could potentially support the growthof axons in the central nervous system. In the present workwe describe the behavior of migrating into the injury dosedregion spinal cord Schwann cells under the influence ofneurotrophic factors - vascular endothelial growth factor(VEGF) and fibroblast growth factor 2 (FGF2), deliveredby direct introduction of «naked» plasmid DNA and bytransplantation of genetically modified human umbilical cordblood mononuclear cells.Using immunohistochemical detection of markers of S100,GFAP, Krox20 and HSP25 identified different phenotypesof migrating into the spinal cord of endogenous Schwanncells. Found that greatest influence on their numbers in theinjury region provides local delivery of genes vegf and fgf2by human umbilical cord blood mononuclear cells. However,the direct introduction of the same plasmid may also bepromising in the case of synthetic platforms that enhanceits transfection activity.

Keywords

vegf / fgf2 / spinal cord injury / Schwann cells / umbilical cord blood cells / plasmid / vegf / fgf2

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