Effect of binase on phorbol myristate acetate-induced apoptosis of human peripheral bloodgranulocytes and monocytes

V A Mironov; F V Shirshikov; N V Kalacheva; G V Cherepnev

doi:10.23868/gc121631

Genes & Cells ›› 2012, Vol. 7 ›› Issue (3) : 108 -111. DOI: 10.23868/gc121631

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Effect of binase on phorbol myristate acetate-induced apoptosis of human peripheral bloodgranulocytes and monocytes

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Abstract

Effect of binase (RNAse of Bacillus intermedius) onphorbol myristate acetate-(PMA)-induced apoptosis of humanperipheral blood granulocytes and monocytes was studied invitro by flow cytometry. Both toxic (400 μg/ml) and nontoxic(40 μg/ml) binase concentrations were tested. The binaseend-point effect was dependent on the target cell populationand the binase concentration. In a granulocyte subset, the400 μg/ml concentration resulted in strongly pronouncedstimulation of PMA-induced apoptosis. In a monocyte subset,the 40 μg/ml concentration developed a protective effect asjudged by an increase in a percantage of viable cell subset andby slowing-down cells transtion from an early to late PMAinducedapoptotic phase.

Keywords

binase / granulocytes / monocytes / phorbol myristate acetate / apoptosis / flow cytometry

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V A Mironov, F V Shirshikov, N V Kalacheva, G V Cherepnev. Effect of binase on phorbol myristate acetate-induced apoptosis of human peripheral bloodgranulocytes and monocytes. Genes & Cells, 2012, 7(3): 108-111 DOI:10.23868/gc121631

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