Uluchshenie dostavki kompleksov plazmidnoy DNKs polikationom v kletki chelovekav prisutstvii bloksopolimerov etilen-i propilenoksida

Abstract

The work is aimed at the study of the effect of novelblock copolymers of ethylene oxide and propylene oxideon the delivery of plasmid DNA and its complexes withcationic polymers into human cells. Tri-functional amphiphilicblock copolymers on the basis of glycerol (LaprolsTM),polyethyleneimine (25 kDa) and commercial transfectionreagent TurboFectTM were tested as delivery systems.TurboFect was found to form more compact and positivelycharged polyplexes with plasmid DNA (pEGFP-N2) andprovided higher expression of GFP in HEK 293 cellscompared to polyethyleneimine. Laprols weakly interactedwith plasmid DNA and did not improve its intracellulardelivery. However they markedly promoted cell transfectionby DNA-polyethyleneimine complexes. Results show thatLaprols exhibit mild cytotoxicity and produce the interest forgene therapeutics delivery into cells.

Keywords

plasmid DNA / polyplexes / polymeric carriers / block copolymers of ethylene oxide and propylene oxide / gene therapy

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