Antitumor RNase (binase) induces the alteration of cellular permeability

Abstract

Some RNases including ones of microbial origin possessantitumor activity, which mechanisms remains unclear. Herewe investigated the first step of RNase action towardseukaryotic cells which is connected with increase of cellpermeability for ions and macromolecules.Using radiological analysis of 45Са2+uptake by Candidayeast and fluorescence imaging of human embryo kidneycells HEK stained by Ca2+-specific Fura-2/АМ day the levelof intracellular Ca2+ under treatment with the RNase ofBacillus intermedius (binase) was studied. Viability of lungcarcinoma epithelial cells A549 treated by binase wasmeasured by WST proliferation kit, stability of erythrocyteswas tested by lysis assay.We have shown that binase induces the permeabilityincrease of lower and higher eukaryotic cells for Ca2+aswell as the increase of protein permeability of A549 cells.Binase treatment protects erythrocytes from osmoticshock.The protective or cytotoxic binase effect followed byincrease of cellular permeability is realized depending on thedell type, where the expression of КСa channels and of certainoncogens, particularly of ras family, is crucial. The obtaineddata supports the significance of the cell permeability increaseas a primary step in the mechanisms of binase-inducedbiological effects.

Keywords

Binase / cytotoxicity / cellular permeability

References

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