Participation of the mononuclear cells of the cord blood in the physiologic regeneration of the rat kidney

T S Yilmaz; A V Mavlikeeva; A A Gumerova; D I Andreeva; I F Fairushina; A A Kiiasov

doi:10.23868/gc121586

Genes & Cells ›› 2012, Vol. 7 ›› Issue (3) : 69 -71. DOI: 10.23868/gc121586

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Participation of the mononuclear cells of the cord blood in the physiologic regeneration of the rat kidney

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Abstract

Nowadays ability to use hematopoietic stem cells fortreatment of various diseases, including kidney pathology,is widely investigated. Umbilical cord blood as the source ofhematopoietic stem cells becomes more and more promising.The purpose of our investigation was to study homing andways of human umbilical cord blood mononuclear cellsdifferentiation in an intact rat kidney. We transplanted humanumbilical cord blood mononuclear cells fraction, enrich withhematopoietic stem cells, into the tail vein of rats. On 2, 5,7 and 14 days after transplantation paraffin kidney sliceswere immunohistochemically stained with antibodies againsthuman leukocyte antigen (HLA-ABC) to study migration anddifferentiation of transplanted cells. Results: HLA-ABC+-cells were revealed in the epithelium of the distal tubule at allexperimental dates. But HLA-ABC was expressed not in eachdistal tubule and not by all tubular cells. We concluded thathuman umbilical cord blood mononuclear cells transplanted intosystemic circulation of rat migrate into the intact kidney andbuilt in the distal tubule epithelium. This data allow to suggestthat distal tubule are stem cell «niche» in the kidney.

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kidney / regeneration / human umbilical cord blood mononuclear cells / transplantation / differentiation

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T S Yilmaz, A V Mavlikeeva, A A Gumerova, D I Andreeva, I F Fairushina, A A Kiiasov. Participation of the mononuclear cells of the cord blood in the physiologic regeneration of the rat kidney. Genes & Cells, 2012, 7(3): 69-71 DOI:10.23868/gc121586

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