Influence of serum antibodies to DNA on theMadin-Darby сanine kidney epithelial cells in vitroin depend of immunoreactive regions of the IgG molecule

Abstract

The data obtained on cell lines MDCK showed that forimplementation of the biological function of antibodies to DNAboth normal and pathological need the participation as Fab- andFc-fragments of molecules of IgG. However, antigen-bindingregion is responsible for the manifestation of the biologicalfunction of SLE antibodies to DNA more immunoreactivethan antibodies in normal and constant region is probablyresponsible for the active conformation of antibody for pathobiologicalfunction of antibodies to DNA in the cell.

Keywords

MDCK / antibodies to DNA / systemic lupus erythematosus / MDCK

References

[1] Арлеевская М. И, Габдулхакова А. Г., Цибулькин А. П.. Диа- гностические и прогностические маркеры ревматоидного артрита с точки зрения патофизиолога. Казань; 2010.

[2] Невзорова, Т. А., Винтер В. Г., Коновалова О. А. и др. Ме- ханизм действия ДНК-гидролизующих АТ к ДНК из крови паци- ентов с системной красной волчанкой. Биохимия 2006; 71 (11): 1238-46. 3. Kolthur-Seetharam Pradeepa U., Gupta N., Narayanaswamy

[3] R. Spatiotemporal organization of AT- and GC-rich DNA and their association with transition proteins TP1 and TP2 in rat condensing spermatids. Histochem. Cytochem. 2009; 57(10): 951-62.

[4] Williams J.M., Colman R., Brookes C.J. et al. Anti-endothelial cell antibodies from lupus patients bind to apoptotic endothelial cells promoting macrophage phagocitosis but do not induce apoptosis. Rheumatology 2005; 44: 879-84.

[5] Madaio M.P., Yanase K., Foster M.H. et al. Nuclear localizing of antibodies. Novel insights into protein traslocation and cellular function. Ann. N. Y. Acad. Sci. 1998; 815: 263-6.

[6] Yung S., Chan T. M. Mycophenolic acid reduces anti-DNA antibody binding and cytokine secretion in renal proximal tubular epithelial cells - implications in lupus nephritis Suppl. 2007; 16: 46-7.

[7] Alarcon-Segovia D., Ruiz-Arguelles A., Fishbein E. Antibody to nuclear ribonucleo-protein penetrates live human mononuclear cells through Fc receptors. Nature. 1978; 271 (640): 67-9.

[8] Vlahakos D., Foster M.H., Ucci A.A. Murine monoclonal anti- DNA antibodies penetrate cells, bind to nuclei, and induce glomerular proliferation and proteinuria in vivo J. Am. Soc. Nephrol. 1992; 2: 1345-54.

[9] Katz J.B., Diamond B.J. Mutational analysis of an autoantibody: differential binding and pathogenicity. Exp. Med. 1994; 180: 925.

[10] Skvortsova Т.Е., Rykova E.Y., Tamkovich S.N. et al. Cell-free and cell-bound circulating DNA in breast tumors: DNA quantification and analysis of tumor-related gene methylation. British J. Cancer. 2006; 94: 1492-5.

[11] Widaa A., Claro T., Foster T.J. et al. Staphylococcus aureus protein A plays a critical role in mediating bone destruction and bone Loss in osteomyelitis. PLoS One 2012; 7(7): 1-9.

RIGHTS & PERMISSIONS

Eco-Vector