The cytofluorimetric characteristics of RNAse influence towards pro- and eucariotic cells

Abstract

Cytotoxic ribonucleases (RNAses) are known to beperspective drugs for cancer therapy. The extension of modelobjects range will give possibility to assess the cytotoxicRNAses selectivity.We estimated the effect of Bacillus intermediusribonuclease (binase) and bovine RNAse A to E. coli K 12 andlung epithelia of cow embryo (LEC) cells. LEC cells apoptosiswas characterized with a double staining with annexin-FITC andpropidium iodide (PI). E. coli K12 cells vitality was estimatevia PI staning.Binase and RNAse A were not cytotoxic to LEC andE. coli K12 cells in investigated concentrations (100 and300 ƒg/ml).Low RNAses toxicity for E. coli allows to suppose thebinase and RNAse A in concentrations, capable to displayantitumor activity, will not to effect the tissues microbialflora during in vivo tests. The lack of apoptosis inducingactivity of binase for LEC cells confirms this RNAse selectivityfor tumor cells.

Keywords

binase / RNAse A / cytotoxic RNAses / apoptosis / selectivity / LEC

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