Gene transfer using new complexes between cardiolipin-like dicationic lipids and plasmid DNA to tumor cells

Abstract

The lipid vesicles of bisamphiphiles cardiolipin-like dicationiclipids (CDL) I-IV were studied for creation of lipoplexes withplasmid DNA of different sizes to obtain stable lipoplexes forgene transfer to gene therapy. Lipoplexes sizes (300±100 nm)and stablity (> 2 hrs) of CDL were sufficient to be used ingene transfer against monolayer and suspension cell cultures.The CDL total cytotoxicity determined by MTT-test was lowercompare to lipofectin as a control. Transfection conditionsagainst tumor cells lines were optimized by lipoplexes of CDLand plasmid DNA. The most efficient transfection for lipoplexesCDL-plasmid DNA was at the lipid-DNA (L/D) ratio equal to 5(for lipofectin, it was 2). For monolayer cell cultures, lipoplexesCDL-I are comparable in terms of transfection efficacy withlipofectin; in the case of suspension culture, their efficiencywas lower by one order of magnitude. It permits a usageof lipoplexes suggested as mediators for gene transfer anddelivery to human tumor cells.

Keywords

dicationic lipids / lipofection / reporter gene / gene transfer / cell culture

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