Polymer-stabilised magnetic nanoparticles do not affect the viability of magnetically-functionalised cells

Abstract

Here we report the synthesis of (poly)allylamine-coatedsuperparamagnetic iron oxide nanoparticles for the surfacemodification of living cells. Magnetic functionalisation of cowembryonic lung cells did not affect the viability of the coatedcells, confluent monolayer formation and proliferation, asdemonstrated using fluorescence and white light microscopy,flow cytometry and MTT assay. Functionalised cells weremagnetically responsive. We believe that the single-stepapproach described here is a novel and potentially promisingway to functionalise mammal cells with magnetic nanoparticlesfor the subsequent applications in cell therapy, directed cellsdelivery and spatial positioning in tissue engineering.

Keywords

iron oxide superparamagnetic nanoparticles / cytotoxicity / cow embryonic lung cells

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