Characteristics of placental multipotent mesenchymal stromal stem cells

Abstract

In this paper we have shown the presence of population of cytokeratyn-7-producing cells in culture of multipotent mesenchymal stromal cells (MMSC] from placenta with immunophenotype CD90 +/CD73 +/CD105 +/HLA -ABD ow/CD34 -/ CD45 -/CD133 -/CD14 -. It was shown by immunocytochemistry of placental MMCS a significant decrease of the population of cytokeratyn-7 positive cells during three passages in vitro. A chimerism of mother cells in cell culture of placental MMSC was established. PCR analysis has detected expression of transcription factors genes which are typical for embryonic and heart stem cells OCT-4 and NKX2-5, and at first the expression of genes sppl, col2a1 and ppar-y2 in cell cultures of placental MMSC with adipogenic, osteogenic and chondrogenic potential in vitro was described. Also we showed the necessity of individual selection of marker genes for differentiation of MMCS from various sources, depending on their initial level of expression.

Keywords

multipotent mesenchymal stromal cells / human placenta / cytokeratin-7 / differentiation

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