Stimulation of rats sciatic nerve post-traumatic regeneration using plasmids expressing vascular endothelialgrowth factor and basic fibroblast growth factor

R F Masgutov; I I Salafutdinov; A A Bogov; A A Trofimova; I G Khannanova; R I Mullin; R R Islamov; Yu A Chelyshev; A A Bogov; A A Rizvanov

doi:10.23868/gc121554

Genes & Cells ›› 2011, Vol. 6 ›› Issue (3) : 67 -70. DOI: 10.23868/gc121554

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Stimulation of rats sciatic nerve post-traumatic regeneration using plasmids expressing vascular endothelialgrowth factor and basic fibroblast growth factor

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Republic Clinical Hospital, KazanKazan (Volga Region) Federal University, KazanKazan State Medical University, Kazan

Republic Clinical Hospital, KazanKazan (Volga Region) Federal University, Kazan

Kazan State Medical University, Kazan

Republic Clinical Hospital, Kazan

Kazan (Volga Region) Federal University, KazanKazan State Medical University, Kazan

Republic Clinical Hospital, KazanKazan (Volga Region) Federal University, KazanKazan State Medical University, Kazan

Republic Clinical Hospital, KazanKazan (Volga Region) Federal University, Kazan

Kazan State Medical University, Kazan

Republic Clinical Hospital, KazanKazan (Volga Region) Federal University, Kazan

Republic Clinical Hospital, Kazan

Kazan (Volga Region) Federal University, KazanKazan State Medical University, Kazan

Republic Clinical Hospital, Kazan

Republic Clinical Hospital, KazanKazan (Volga Region) Federal University, KazanKazan State Medical University, Kazan

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Abstract

The development of effective treatments for patientswith peripheral nerve injury is an urgent task of biomedicine.«Gold» standard in restoring the integrity of nerve conduits isauto-nerve transplantation in which a peripheral nerve defectis corrected with autologous nerve graft. Here we proposea method for stimulating revascularization and regenerationof auto-nerve graft by a local injection of plasmid pBud-VEGFFGF2,expressing vascular endothelial growth factor (VEGF)and basic fibroblast growth factor (FGF2). It is shown thatdirect injection of plasmid pBud-VEGF-FGF2 in the proximaland distal segments of nerve, as well as in the auto-nervegraft, stimulates the regeneration of the rats sciatic nerveand restores motor activity.

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R F Masgutov, I I Salafutdinov, A A Bogov, A A Trofimova, I G Khannanova, R I Mullin, R R Islamov, Yu A Chelyshev, A A Bogov, A A Rizvanov. Stimulation of rats sciatic nerve post-traumatic regeneration using plasmids expressing vascular endothelialgrowth factor and basic fibroblast growth factor. Genes & Cells, 2011, 6(3): 67-70 DOI:10.23868/gc121554

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References

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