Kompozitsionnye matritsy na osnove khitina i khitozana dlya kul'tivirovaniya kletok kozhi cheloveka

E F Panarin; L A Nud'ga; V A Petrova; A M Bochek; I V Goffman; Yu G Baklagina; N N Saprykina; M I Blinova; N M Yudintseva; O G Spichkina; L V Kukhareva; I A Samusenko; G P Pinaev

doi:10.23868/gc121498

Genes & Cells ›› 2010, Vol. 5 ›› Issue (1) : 65 -73. DOI: 10.23868/gc121498

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Kompozitsionnye matritsy na osnove khitina i khitozana dlya kul'tivirovaniya kletok kozhi cheloveka

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the Institute of Macromolecular Compounds, RAS, Saint-Petersburg 2 the Institute of Cytology, RAS, Saint-Petersburg

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

the Institute of Cytology, RAS, Saint-Petersburg

A.M. Nikiforov National Center of Urgent and Radiation Medicine, EMERCOM of Russia, Saint-Petersburg

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg 2 the Institute of Cytology, RAS, Saint-Petersburg

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

the Institute of Cytology, RAS, Saint-Petersburg

A.M. Nikiforov National Center of Urgent and Radiation Medicine, EMERCOM of Russia, Saint-Petersburg

the Institute of Cytology, RAS, Saint-Petersburg

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Abstract

A set of composite films based on chitosan or chitosan-collagen mixtures are developed to be used as the resorbable matrices for fibroblastes and keratinocytes cultivation, and optimal conditions of their formation are precised. 3D porous matrices are obtained to insure the simultaneous cultivation of both types of cells. The composite materials prepared by this method in forms of films and sponges were characterized and then tested as the matrices for cell cultivation to obtain the transplantats for wound healing. Both in vitro and in vivo tests of all materials under study have evidenced the absence of any toxic actions on the cultivated cells and on the tissues of organism. The matrices are stable in the conditions of cultivation and resorb completely in in vivo conditions in 10 days after the transplantation.

Keywords

polysaccharides / chitin / chitosan / collagen / sponge / membranes / fibroblasts / keratinocytes / culturing

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E F Panarin, L A Nud'ga, V A Petrova, A M Bochek, I V Goffman, Yu G Baklagina, N N Saprykina, M I Blinova, N M Yudintseva, O G Spichkina, L V Kukhareva, I A Samusenko, G P Pinaev. Kompozitsionnye matritsy na osnove khitina i khitozana dlya kul'tivirovaniya kletok kozhi cheloveka. Genes & Cells, 2010, 5(1): 65-73 DOI:10.23868/gc121498

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References

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